Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4466297..4466836 Replicon chromosome
Accession NZ_CP038292
Organism Escherichia coli O157:H7 strain TB21-1

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag E4U65_RS22580 Protein ID WP_001303998.1
Coordinates 4466576..4466836 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag E4U65_RS22575 Protein ID WP_000729705.1
Coordinates 4466297..4466557 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U65_RS22555 4462051..4462836 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
E4U65_RS22560 4462781..4464520 + 1740 WP_001301901.1 flagellar biosynthesis protein FlhA -
E4U65_RS22565 4464665..4465162 - 498 Protein_4420 REP-associated tyrosine transposase RayT -
E4U65_RS22570 4465338..4466111 - 774 WP_000543891.1 C40 family peptidase -
E4U65_RS22575 4466297..4466557 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
E4U65_RS22580 4466576..4466836 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
E4U65_RS22585 4466992..4467732 + 741 WP_001225679.1 murein L,D-transpeptidase -
E4U65_RS22590 4467703..4468470 - 768 WP_001301698.1 class II glutamine amidotransferase -
E4U65_RS22595 4468575..4469153 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T121143 WP_001303998.1 NZ_CP038292:4466576-4466836 [Escherichia coli O157:H7]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T121143 NZ_CP038292:4466576-4466836 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT121143 WP_000729705.1 NZ_CP038292:4466297-4466557 [Escherichia coli O157:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT121143 NZ_CP038292:4466297-4466557 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References