Detailed information of TA system TA121137

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3516061..3516286
• Replicon: chromosome
• Accession: NZ_CP038292
• Organism: Escherichia coli O157:H7 strain TB21-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U65_RS18010
• Protein ID: WP_000813263.1
• Coordinates: 3516061..3516216 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3516228..3516286 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U65_RS17975	3511515..3512228	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4U65_RS17980	3512366..3512562	-	197	Protein_3520	TrmB family transcriptional regulator	-
E4U65_RS17985	3512849..3513667	-	819	WP_171878112.1	CPBP family intramembrane metalloprotease	-
E4U65_RS17990	3513819..3514190	-	372	WP_000090264.1	antitermination protein	-
E4U65_RS17995	3514180..3514551	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4U65_RS18000	3514564..3515613	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4U65_RS18005	3515615..3515893	-	279	WP_001341388.1	hypothetical protein	-
E4U65_RS18010	3516061..3516216	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3516228..3516286	+	59	-	-	Antitoxin
E4U65_RS18015	3516318..3516455	+	138	WP_000955173.1	hypothetical protein	-
E4U65_RS18020	3516821..3517594	+	774	WP_000160654.1	alpha/beta hydrolase	-
E4U65_RS18025	3517946..3518359	-	414	WP_001151233.1	DUF977 family protein	-
E4U65_RS18030	3518375..3519145	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U65_RS18035	3519167..3519913	-	747	WP_000788751.1	ATP-binding protein	-
E4U65_RS18040	3519920..3521011	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3481540..3530674	49134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T121137 WP_000813263.1 NZ_CP038292:c3516216-3516061 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T121137 NZ_CP038292:c3516216-3516061 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121137 NZ_CP038292:3516228-3516286 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin