Detailed information of TA system TA121133

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2848720..2848945
• Replicon: chromosome
• Accession: NZ_CP038292
• Organism: Escherichia coli O157:H7 strain TB21-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U65_RS14625
• Protein ID: WP_000935259.1
• Coordinates: 2848733..2848945 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2848720..2848778 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U65_RS14575	2843791..2844033	+	243	WP_000747948.1	hypothetical protein	-
E4U65_RS14580	2844017..2844442	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U65_RS14585	2844511..2845548	+	1038	WP_136760546.1	phage replisome organizer	-
E4U65_RS14590	2845541..2846002	+	462	WP_000139447.1	replication protein	-
E4U65_RS14595	2846036..2846752	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U65_RS14600	2846785..2847066	+	282	WP_000603384.1	DNA-binding protein	-
E4U65_RS14605	2847063..2847290	+	228	WP_000699809.1	hypothetical protein	-
E4U65_RS14610	2847283..2847594	+	312	WP_001289673.1	hypothetical protein	-
E4U65_RS14615	2847722..2847940	+	219	WP_000683609.1	DUF4014 family protein	-
E4U65_RS14620	2847942..2848499	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2848720..2848778	-	59	-	-	Antitoxin
E4U65_RS14625	2848733..2848945	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U65_RS14630	2849065..2849409	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U65_RS14635	2849531..2849803	+	273	WP_000191872.1	hypothetical protein	-
E4U65_RS14640	2849805..2850854	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U65_RS14645	2850867..2851172	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U65_RS14650	2851235..2851789	+	555	WP_000640035.1	DUF1133 family protein	-
E4U65_RS14655	2852014..2852211	+	198	WP_000917763.1	hypothetical protein	-
E4U65_RS14660	2852347..2853060	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U65_RS14675	2853511..2853942	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7'	2785165..2886059	100894

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121133 WP_000935259.1 NZ_CP038292:2848733-2848945 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121133 NZ_CP038292:2848733-2848945 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121133 NZ_CP038292:c2848778-2848720 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin