Detailed information of TA system TA121111

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ldrD-sokW/Ldr(toxin)
• Location: 277125..277346
• Replicon: chromosome
• Accession: NZ_CP038292
• Organism: Escherichia coli O157:H7 strain TB21-1

Toxin (Protein)

• Gene name: ldrD
• Uniprot ID: S1NWX0
• Locus tag: E4U65_RS01320
• Protein ID: WP_001295224.1
• Coordinates: 277239..277346 (+)
• Length: 36 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	277125..277190 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U65_RS01300	272565..273467	+	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
E4U65_RS01305	273478..274461	+	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	-
E4U65_RS01310	274458..275462	+	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
E4U65_RS01315	275492..276763	-	1272	WP_001301684.1	amino acid permease	-
-	277125..277190	-	66	NuclAT_16	-	Antitoxin
-	277125..277190	-	66	NuclAT_16	-	Antitoxin
-	277125..277190	-	66	NuclAT_16	-	Antitoxin
-	277125..277190	-	66	NuclAT_16	-	Antitoxin
-	277125..277190	-	66	NuclAT_21	-	Antitoxin
-	277125..277190	-	66	NuclAT_21	-	Antitoxin
-	277125..277190	-	66	NuclAT_21	-	Antitoxin
-	277125..277190	-	66	NuclAT_21	-	Antitoxin
E4U65_RS01320	277239..277346	+	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	Toxin
E4U65_RS01325	277433..279112	-	1680	WP_000191592.1	cellulose biosynthesis protein BcsG	-
E4U65_RS01330	279109..279300	-	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
E4U65_RS01335	279297..280868	-	1572	WP_001204920.1	cellulose biosynthesis protein BcsE	-
E4U65_RS01340	281141..281329	+	189	WP_001063316.1	YhjR family protein	-
E4U65_RS01345	281341..281538	+	198	WP_000279508.1	AAA family ATPase	-
E4U65_RS01350	281557..282093	+	537	WP_001270903.1	cellulose synthase operon protein YhjQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 3882.70 Da
• Isoelectric Point: 9.0157

>T121111 WP_001295224.1 NZ_CP038292:277239-277346 [Escherichia coli O157:H7]
MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK

Nucleotide

• Length: 108 bp

>T121111 NZ_CP038292:277239-277346 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT121111 NZ_CP038292:c277190-277125 [Escherichia coli O157:H7]
GTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A4P7TSJ7

Antitoxin