Detailed information of TA system TA121083

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2723746..2723971
• Replicon: chromosome
• Accession: NZ_CP038290
• Organism: Escherichia coli O157:H7 strain TX 265-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U08_RS14010
• Protein ID: WP_000967408.1
• Coordinates: 2723759..2723971 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2723746..2723804 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U08_RS13965	2718999..2719952	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
E4U08_RS13970	2719959..2720699	+	741	WP_000790456.1	ATP-binding protein	-
E4U08_RS13975	2720729..2721499	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
E4U08_RS13980	2721515..2721910	+	396	WP_001118161.1	DUF977 family protein	-
E4U08_RS13985	2721967..2722323	+	357	WP_001302146.1	hypothetical protein	-
E4U08_RS13990	2722372..2722584	+	213	WP_000063625.1	hypothetical protein	-
E4U08_RS13995	2722620..2722991	+	372	WP_000137941.1	hypothetical protein	-
E4U08_RS14000	2722988..2723350	+	363	WP_000610379.1	DUF551 domain-containing protein	-
E4U08_RS14005	2723466..2723570	+	105	WP_001278450.1	hypothetical protein	-
-	2723746..2723804	-	59	-	-	Antitoxin
E4U08_RS14010	2723759..2723971	+	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U08_RS14015	2724268..2724435	+	168	WP_000998188.1	hypothetical protein	-
E4U08_RS14020	2724501..2724779	+	279	WP_001304183.1	hypothetical protein	-
E4U08_RS14025	2724781..2725830	+	1050	Protein_2745	DUF968 domain-containing protein	-
E4U08_RS14030	2725843..2726217	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E4U08_RS14035	2726214..2727035	+	822	WP_000762904.1	antitermination protein	-
E4U08_RS14040	2727262..2727459	+	198	WP_000917735.1	hypothetical protein	-
E4U08_RS14045	2727610..2728668	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / espM1 / ospG	2607126..2795360	188234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T121083 WP_000967408.1 NZ_CP038290:2723759-2723971 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T121083 NZ_CP038290:2723759-2723971 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121083 NZ_CP038290:c2723804-2723746 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin