Detailed information of TA system TA121081

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2677870..2678095
• Replicon: chromosome
• Accession: NZ_CP038290
• Organism: Escherichia coli O157:H7 strain TX 265-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U08_RS13695
• Protein ID: WP_000882662.1
• Coordinates: 2677883..2678095 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2677870..2677928 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U08_RS13655	2673021..2673428	-	408	WP_001003379.1	helix-turn-helix domain-containing protein	-
E4U08_RS13660	2673506..2673733	+	228	WP_000476986.1	transcriptional regulator	-
E4U08_RS13665	2673717..2674268	+	552	WP_171876995.1	hypothetical protein	-
E4U08_RS13670	2674240..2675280	+	1041	WP_000020565.1	DNA-binding protein	-
E4U08_RS13675	2675192..2675734	+	543	WP_157837342.1	replication protein	-
E4U08_RS13680	2675769..2676527	+	759	WP_000537579.1	DUF1627 domain-containing protein	-
E4U08_RS13685	2676587..2676772	+	186	WP_000215514.1	hypothetical protein	-
E4U08_RS13690	2677120..2677668	+	549	WP_000211435.1	ORF6N domain-containing protein	-
-	2677870..2677928	-	59	-	-	Antitoxin
E4U08_RS13695	2677883..2678095	+	213	WP_000882662.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U08_RS13700	2678198..2678515	-	318	WP_000042395.1	DNA-binding transcriptional regulator	-
E4U08_RS13705	2678508..2678879	-	372	WP_001217944.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4U08_RS13710	2679103..2679330	+	228	WP_001452497.1	hypothetical protein	-
E4U08_RS13715	2679384..2679653	+	270	WP_024177817.1	hypothetical protein	-
E4U08_RS13720	2679655..2680704	+	1050	WP_001265189.1	DUF968 domain-containing protein	-
E4U08_RS13725	2680717..2681091	+	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
E4U08_RS13730	2681088..2681909	+	822	WP_000762902.1	antitermination protein	-
E4U08_RS13735	2682136..2682333	+	198	WP_000917733.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / espM1 / ospG	2607126..2795360	188234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7845.45 Da
• Isoelectric Point: 8.5044

>T121081 WP_000882662.1 NZ_CP038290:2677883-2678095 [Escherichia coli O157:H7]
MLCTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T121081 NZ_CP038290:2677883-2678095 [Escherichia coli O157:H7]
ATGCTTTGCACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATTGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121081 NZ_CP038290:c2677928-2677870 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Structures

Antitoxin