Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3296675..3297470 Replicon chromosome
Accession NZ_CP038287
Organism Escherichia coli O157:H7 strain TX 376-2

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E4U64_RS16835 Protein ID WP_000854914.1
Coordinates 3296675..3297049 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID -
Locus tag E4U64_RS16840 Protein ID WP_001416641.1
Coordinates 3297096..3297470 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U64_RS16795 3291679..3292170 - 492 WP_001301587.1 DUF1097 domain-containing protein -
E4U64_RS16800 3292272..3292826 - 555 WP_001001917.1 molecular chaperone YcdY -
E4U64_RS16805 3292850..3293587 - 738 WP_000283664.1 zinc-binding phosphatase -
E4U64_RS16810 3293642..3294580 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
E4U64_RS16820 3295051..3295893 - 843 WP_001280481.1 DUF4942 domain-containing protein -
E4U64_RS16825 3295978..3296175 - 198 WP_000839282.1 DUF957 domain-containing protein -
E4U64_RS16830 3296187..3296678 - 492 WP_000976853.1 hypothetical protein -
E4U64_RS16835 3296675..3297049 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E4U64_RS16840 3297096..3297470 - 375 WP_001416641.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E4U64_RS16845 3297633..3297854 - 222 WP_001220314.1 DUF987 domain-containing protein -
E4U64_RS16850 3297917..3298393 - 477 WP_001186738.1 RadC family protein -
E4U64_RS16855 3298409..3298894 - 486 WP_000214398.1 antirestriction protein -
E4U64_RS16860 3298985..3299803 - 819 WP_001234682.1 DUF945 domain-containing protein -
E4U64_RS16865 3300143..3301213 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
E4U64_RS16870 3301210..3302115 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3288061..3298894 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T121049 WP_000854914.1 NZ_CP038287:c3297049-3296675 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T121049 NZ_CP038287:c3297049-3296675 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13778.61 Da        Isoelectric Point: 6.6249

>AT121049 WP_001416641.1 NZ_CP038287:c3297470-3297096 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGFCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT121049 NZ_CP038287:c3297470-3297096 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTTCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5


Antitoxin

Source ID Structure

References