Detailed information of TA system TA121046

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2635038..2635263
• Replicon: chromosome
• Accession: NZ_CP038287
• Organism: Escherichia coli O157:H7 strain TX 376-2

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U64_RS13335
• Protein ID: WP_000935259.1
• Coordinates: 2635051..2635263 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2635038..2635096 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U64_RS13285	2630103..2630345	+	243	WP_000747948.1	hypothetical protein	-
E4U64_RS13290	2630329..2630754	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U64_RS13295	2630823..2631866	+	1044	WP_128484576.1	phage replisome organizer	-
E4U64_RS13300	2631859..2632320	+	462	WP_000139447.1	replication protein	-
E4U64_RS13305	2632354..2633070	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U64_RS13310	2633103..2633384	+	282	WP_000603384.1	DNA-binding protein	-
E4U64_RS13315	2633381..2633608	+	228	WP_000699809.1	hypothetical protein	-
E4U64_RS13320	2633601..2633912	+	312	WP_001289673.1	hypothetical protein	-
E4U64_RS13325	2634040..2634258	+	219	WP_000683609.1	DUF4014 family protein	-
E4U64_RS13330	2634260..2634817	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2635038..2635096	-	59	-	-	Antitoxin
E4U64_RS13335	2635051..2635263	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U64_RS13340	2635383..2635727	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U64_RS13345	2635849..2636121	+	273	WP_000191872.1	hypothetical protein	-
E4U64_RS13350	2636123..2637172	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U64_RS13355	2637185..2637490	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U64_RS13360	2637553..2638107	+	555	WP_000640035.1	DUF1133 family protein	-
E4U64_RS13365	2638332..2638529	+	198	WP_000917763.1	hypothetical protein	-
E4U64_RS13370	2638665..2639378	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U64_RS13385	2639829..2640260	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2515004..2679267	164263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121046 WP_000935259.1 NZ_CP038287:2635051-2635263 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121046 NZ_CP038287:2635051-2635263 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121046 NZ_CP038287:c2635096-2635038 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin