Detailed information of TA system TA121012

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3555787..3556012
• Replicon: chromosome
• Accession: NZ_CP038284
• Organism: Escherichia coli O157:H7 strain YB14-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U79_RS18245
• Protein ID: WP_000813263.1
• Coordinates: 3555787..3555942 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3555954..3556012 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U79_RS18210	3551241..3551954	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4U79_RS18215	3552092..3552288	-	197	Protein_3567	TrmB family transcriptional regulator	-
E4U79_RS18220	3552575..3553393	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E4U79_RS18225	3553545..3553916	-	372	WP_000090264.1	antitermination protein	-
E4U79_RS18230	3553906..3554277	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4U79_RS18235	3554290..3555339	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4U79_RS18240	3555341..3555619	-	279	WP_001341388.1	hypothetical protein	-
E4U79_RS18245	3555787..3555942	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3555954..3556012	+	59	-	-	Antitoxin
E4U79_RS18250	3556044..3556181	+	138	WP_000955173.1	hypothetical protein	-
E4U79_RS18255	3556547..3557320	+	774	WP_000160654.1	alpha/beta hydrolase	-
E4U79_RS18260	3557672..3558085	-	414	WP_001151233.1	DUF977 family protein	-
E4U79_RS18265	3558101..3558871	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U79_RS18270	3558893..3559639	-	747	WP_000788751.1	ATP-binding protein	-
E4U79_RS18275	3559646..3560737	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T121012 WP_000813263.1 NZ_CP038284:c3555942-3555787 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T121012 NZ_CP038284:c3555942-3555787 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121012 NZ_CP038284:3555954-3556012 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin