Detailed information of TA system TA121009

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2995994..2996219
• Replicon: chromosome
• Accession: NZ_CP038284
• Organism: Escherichia coli O157:H7 strain YB14-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U79_RS15100
• Protein ID: WP_000967408.1
• Coordinates: 2995994..2996206 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2996161..2996219 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U79_RS15065	2991280..2992338	-	1059	WP_000935552.1	site-specific DNA-methyltransferase	-
E4U79_RS15070	2992489..2992686	-	198	WP_000917735.1	hypothetical protein	-
E4U79_RS15075	2992913..2993734	-	822	WP_000762909.1	antitermination protein	-
E4U79_RS15080	2993731..2994105	-	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E4U79_RS15085	2994118..2995167	-	1050	WP_001265167.1	DUF968 domain-containing protein	-
E4U79_RS15090	2995169..2995447	-	279	WP_032156506.1	hypothetical protein	-
E4U79_RS15095	2995517..2995777	-	261	WP_077625879.1	hypothetical protein	-
E4U79_RS15100	2995994..2996206	-	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2996161..2996219	+	59	-	-	Antitoxin
E4U79_RS15105	2996560..2997354	+	795	WP_000373318.1	protein kinase	-
E4U79_RS15110	2997338..2998054	+	717	WP_000789359.1	protein phosphatase 2C domain-containing protein	-
E4U79_RS15115	2998314..2998487	-	174	WP_001224661.1	hypothetical protein	-
E4U79_RS15120	2998583..2998939	-	357	WP_000403785.1	hypothetical protein	-
E4U79_RS15125	2999012..2999392	-	381	WP_001118156.1	DUF977 family protein	-
E4U79_RS15130	2999408..3000178	-	771	WP_000450846.1	DUF1627 domain-containing protein	-
E4U79_RS15135	3000212..3000754	-	543	WP_157837342.1	replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG / paa	2962650..3060716	98066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T121009 WP_000967408.1 NZ_CP038284:c2996206-2995994 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T121009 NZ_CP038284:c2996206-2995994 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121009 NZ_CP038284:2996161-2996219 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin