Detailed information of TA system TA121006

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2637409..2637634
• Replicon: chromosome
• Accession: NZ_CP038284
• Organism: Escherichia coli O157:H7 strain YB14-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U79_RS13375
• Protein ID: WP_000935259.1
• Coordinates: 2637422..2637634 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2637409..2637467 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U79_RS13325	2632474..2632716	+	243	WP_000747948.1	hypothetical protein	-
E4U79_RS13330	2632700..2633125	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E4U79_RS13335	2633194..2634237	+	1044	WP_001262402.1	hypothetical protein	-
E4U79_RS13340	2634230..2634691	+	462	WP_000139447.1	replication protein	-
E4U79_RS13345	2634725..2635441	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4U79_RS13350	2635474..2635755	+	282	WP_000603384.1	DNA-binding protein	-
E4U79_RS13355	2635752..2635979	+	228	WP_000699809.1	hypothetical protein	-
E4U79_RS13360	2635972..2636283	+	312	WP_001289673.1	hypothetical protein	-
E4U79_RS13365	2636411..2636629	+	219	WP_137049466.1	DUF4014 family protein	-
E4U79_RS13370	2636631..2637188	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2637409..2637467	-	59	-	-	Antitoxin
E4U79_RS13375	2637422..2637634	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U79_RS13380	2637754..2638098	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4U79_RS13385	2638220..2638492	+	273	WP_000191872.1	hypothetical protein	-
E4U79_RS13390	2638494..2639543	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4U79_RS13395	2639556..2639861	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4U79_RS13400	2639924..2640478	+	555	WP_000640035.1	DUF1133 family protein	-
E4U79_RS13405	2640703..2640900	+	198	WP_000917763.1	hypothetical protein	-
E4U79_RS13410	2641037..2641750	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4U79_RS13425	2642205..2642633	+	429	WP_001303509.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2518443..2674750	156307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T121006 WP_000935259.1 NZ_CP038284:2637422-2637634 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T121006 NZ_CP038284:2637422-2637634 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121006 NZ_CP038284:c2637467-2637409 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin