Detailed information of TA system TA121004

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2589340..2589565
• Replicon: chromosome
• Accession: NZ_CP038284
• Organism: Escherichia coli O157:H7 strain YB14-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U79_RS13065
• Protein ID: WP_000967408.1
• Coordinates: 2589353..2589565 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2589340..2589398 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U79_RS13020	2584593..2585546	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
E4U79_RS13025	2585553..2586293	+	741	WP_000790456.1	ATP-binding protein	-
E4U79_RS13030	2586323..2587093	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
E4U79_RS13035	2587109..2587504	+	396	WP_001118161.1	DUF977 family protein	-
E4U79_RS13040	2587561..2587917	+	357	WP_001302146.1	hypothetical protein	-
E4U79_RS13045	2587966..2588178	+	213	WP_000063625.1	hypothetical protein	-
E4U79_RS13050	2588214..2588585	+	372	WP_000137941.1	hypothetical protein	-
E4U79_RS13055	2588582..2588944	+	363	WP_000610379.1	DUF551 domain-containing protein	-
E4U79_RS13060	2589060..2589164	+	105	WP_001278450.1	hypothetical protein	-
-	2589340..2589398	-	59	-	-	Antitoxin
E4U79_RS13065	2589353..2589565	+	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U79_RS13070	2589862..2590029	+	168	WP_000998188.1	hypothetical protein	-
E4U79_RS13075	2590095..2590373	+	279	WP_001304183.1	hypothetical protein	-
E4U79_RS13080	2590375..2591424	+	1050	Protein_2554	DUF968 domain-containing protein	-
E4U79_RS13085	2591437..2591811	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E4U79_RS13090	2591808..2592629	+	822	WP_000762904.1	antitermination protein	-
E4U79_RS13095	2592856..2593053	+	198	WP_000917735.1	hypothetical protein	-
E4U79_RS13100	2593204..2594262	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2518443..2674750	156307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T121004 WP_000967408.1 NZ_CP038284:2589353-2589565 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T121004 NZ_CP038284:2589353-2589565 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121004 NZ_CP038284:c2589398-2589340 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin