Detailed information of TA system TA120979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4438513..4439207
• Replicon: chromosome
• Accession: NZ_CP038282
• Organism: Escherichia coli O157:H7 strain F8492

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: E4T53_RS22430
• Protein ID: WP_001263495.1
• Coordinates: 4438513..4438911 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E4T53_RS22435
• Protein ID: WP_000554757.1
• Coordinates: 4438914..4439207 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4434100..4434180	-	81	NuclAT_11	-	-
-	4434100..4434180	-	81	NuclAT_11	-	-
-	4434100..4434180	-	81	NuclAT_11	-	-
-	4434100..4434180	-	81	NuclAT_11	-	-
E4T53_RS22405	4434776..4435234	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E4T53_RS22410	4435495..4436952	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
E4T53_RS22415	4437009..4437566	-	558	WP_023438539.1	peptide chain release factor H	-
E4T53_RS22420	4437478..4437744	-	267	WP_001303804.1	hypothetical protein	-
E4T53_RS22425	4438051..4438503	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
E4T53_RS22430	4438513..4438911	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E4T53_RS22435	4438914..4439207	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E4T53_RS22440	4439259..4440314	-	1056	WP_001226186.1	DNA polymerase IV	-
E4T53_RS22445	4440385..4441170	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
E4T53_RS22450	4441115..4442854	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
E4T53_RS22455	4442999..4443496	-	498	Protein_4401	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4398094..4444445	46351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T120979 WP_001263495.1 NZ_CP038282:c4438911-4438513 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T120979 NZ_CP038282:c4438911-4438513 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT120979 WP_000554757.1 NZ_CP038282:c4439207-4438914 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT120979 NZ_CP038282:c4439207-4438914 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6