Detailed information of TA system TA120974

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3549653..3549878
• Replicon: chromosome
• Accession: NZ_CP038282
• Organism: Escherichia coli O157:H7 strain F8492

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4T53_RS18305
• Protein ID: WP_000813263.1
• Coordinates: 3549653..3549808 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3549820..3549878 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4T53_RS18270	3545107..3545820	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4T53_RS18275	3545958..3546154	-	197	Protein_3582	TrmB family transcriptional regulator	-
E4T53_RS18280	3546441..3547259	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E4T53_RS18285	3547411..3547782	-	372	WP_000090264.1	antitermination protein	-
E4T53_RS18290	3547772..3548143	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4T53_RS18295	3548156..3549205	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4T53_RS18300	3549207..3549485	-	279	WP_001341388.1	hypothetical protein	-
E4T53_RS18305	3549653..3549808	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3549820..3549878	+	59	-	-	Antitoxin
E4T53_RS18310	3549910..3550047	+	138	WP_000955173.1	hypothetical protein	-
E4T53_RS18315	3550413..3551186	+	774	WP_000160654.1	alpha/beta hydrolase	-
E4T53_RS18320	3551538..3551951	-	414	WP_001151233.1	DUF977 family protein	-
E4T53_RS18325	3551967..3552737	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4T53_RS18330	3552759..3553505	-	747	WP_000788751.1	ATP-binding protein	-
E4T53_RS18335	3553512..3554603	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T120974 WP_000813263.1 NZ_CP038282:c3549808-3549653 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T120974 NZ_CP038282:c3549808-3549653 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT120974 NZ_CP038282:3549820-3549878 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin