Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3340923..3341718 Replicon chromosome
Accession NZ_CP038282
Organism Escherichia coli O157:H7 strain F8492

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E4T53_RS17220 Protein ID WP_000854914.1
Coordinates 3340923..3341297 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag E4T53_RS17225 Protein ID WP_001280954.1
Coordinates 3341344..3341718 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4T53_RS17180 3335927..3336418 - 492 WP_001301587.1 DUF1097 domain-containing protein -
E4T53_RS17185 3336520..3337074 - 555 WP_001001917.1 molecular chaperone YcdY -
E4T53_RS17190 3337098..3337835 - 738 WP_000283664.1 zinc-binding phosphatase -
E4T53_RS17195 3337890..3338828 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
E4T53_RS17205 3339299..3340141 - 843 WP_001280481.1 DUF4942 domain-containing protein -
E4T53_RS17210 3340226..3340423 - 198 WP_000839282.1 DUF957 domain-containing protein -
E4T53_RS17215 3340435..3340926 - 492 WP_000976853.1 hypothetical protein -
E4T53_RS17220 3340923..3341297 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E4T53_RS17225 3341344..3341718 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E4T53_RS17230 3341881..3342102 - 222 WP_001220314.1 DUF987 domain-containing protein -
E4T53_RS17235 3342165..3342641 - 477 WP_001186738.1 RadC family protein -
E4T53_RS17240 3342657..3343142 - 486 WP_000214398.1 antirestriction protein -
E4T53_RS17245 3343233..3344051 - 819 WP_001234682.1 DUF945 domain-containing protein -
E4T53_RS17250 3344391..3345461 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
E4T53_RS17255 3345458..3346363 - 906 WP_171878027.1 chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3332309..3343142 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T120973 WP_000854914.1 NZ_CP038282:c3341297-3340923 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T120973 NZ_CP038282:c3341297-3340923 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT120973 WP_001280954.1 NZ_CP038282:c3341718-3341344 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT120973 NZ_CP038282:c3341718-3341344 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References