Detailed information of TA system TA120971

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2981327..2981552
• Replicon: chromosome
• Accession: NZ_CP038282
• Organism: Escherichia coli O157:H7 strain F8492

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4T53_RS15070
• Protein ID: WP_000935259.1
• Coordinates: 2981327..2981539 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2981494..2981552 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4T53_RS15020	2976330..2976761	-	432	WP_001302123.1	tellurite resistance protein	-
E4T53_RS15035	2977212..2977925	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E4T53_RS15040	2978061..2978258	-	198	WP_000917763.1	hypothetical protein	-
E4T53_RS15045	2978483..2979037	-	555	WP_000640035.1	DUF1133 family protein	-
E4T53_RS15050	2979100..2979405	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E4T53_RS15055	2979418..2980467	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
E4T53_RS15060	2980469..2980741	-	273	WP_000191872.1	hypothetical protein	-
E4T53_RS15065	2980863..2981207	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E4T53_RS15070	2981327..2981539	-	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2981494..2981552	+	59	-	-	Antitoxin
E4T53_RS15075	2981773..2982330	-	558	WP_000104474.1	DUF551 domain-containing protein	-
E4T53_RS15080	2982332..2982550	-	219	WP_000683609.1	DUF4014 family protein	-
E4T53_RS15085	2982678..2982989	-	312	WP_001289673.1	hypothetical protein	-
E4T53_RS15090	2982982..2983209	-	228	WP_000699809.1	hypothetical protein	-
E4T53_RS15095	2983206..2983487	-	282	WP_000603384.1	DNA-binding protein	-
E4T53_RS15100	2983520..2984236	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
E4T53_RS15105	2984270..2984731	-	462	WP_000139447.1	replication protein	-
E4T53_RS15110	2984724..2985767	-	1044	WP_001262402.1	hypothetical protein	-
E4T53_RS15115	2985836..2986261	-	426	WP_000693878.1	Rha family transcriptional regulator	-
E4T53_RS15120	2986245..2986487	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / paa	2944226..3044829	100603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T120971 WP_000935259.1 NZ_CP038282:c2981539-2981327 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T120971 NZ_CP038282:c2981539-2981327 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT120971 NZ_CP038282:2981494-2981552 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin