Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 7700..8424 Replicon plasmid pWLK-107717
Accession NZ_CP038276
Organism Raoultella ornithinolytica strain WLK218

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag E4K08_RS00705 Protein ID WP_076752146.1
Coordinates 8113..8424 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E4K08_RS00700 Protein ID WP_076752145.1
Coordinates 7700..8116 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4K08_RS00660 3219..3695 + 477 WP_076752132.1 transglycosylase SLT domain-containing protein -
E4K08_RS00665 3733..4263 - 531 WP_076752134.1 antirestriction protein -
E4K08_RS00670 4455..4685 + 231 WP_076752135.1 hypothetical protein -
E4K08_RS00680 4889..5722 - 834 WP_076752137.1 N-6 DNA methylase -
E4K08_RS00685 5769..5999 - 231 WP_076752138.1 hypothetical protein -
E4K08_RS00690 6088..6435 - 348 WP_076752140.1 hypothetical protein -
E4K08_RS00695 6538..6810 - 273 WP_137055850.1 hypothetical protein -
E4K08_RS00700 7700..8116 - 417 WP_076752145.1 helix-turn-helix domain-containing protein Antitoxin
E4K08_RS00705 8113..8424 - 312 WP_076752146.1 type II toxin-antitoxin system HigB family toxin Toxin
E4K08_RS00710 8574..8786 - 213 WP_076752148.1 hypothetical protein -
E4K08_RS00715 8894..9220 - 327 WP_076752150.1 hypothetical protein -
E4K08_RS00720 10079..10237 - 159 WP_076752152.1 type I toxin-antitoxin system Hok family toxin -
E4K08_RS00725 10579..11064 - 486 WP_076752153.1 GNAT family N-acetyltransferase -
E4K08_RS00730 11052..11318 - 267 WP_012540086.1 DUF1778 domain-containing protein -
E4K08_RS00735 11619..11942 + 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
E4K08_RS00740 11944..12657 + 714 WP_013087170.1 arsenical resistance protein ArsH -
E4K08_RS00745 12666..13211 + 546 WP_047365773.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..107717 107717


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Antitoxin HigA

(5-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12510.31 Da        Isoelectric Point: 9.9242

>T120932 WP_076752146.1 NZ_CP038276:c8424-8113 [Raoultella ornithinolytica]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFNSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Download         Length: 312 bp

>T120932 NZ_CP038276:c8424-8113 [Raoultella ornithinolytica]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCAGCGGCCATCGATGACACATA
TCGTGTACTAAAGCGGGTTGTAGCAAAAAAACCGGATGAGCTAAAGCGATACTTTAATAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGCGGCAATAACCTCAGAATGATGTTTTTTGCTGATTTCGAGCGCGGAAAGATA
TTCGTCAAACACATTACCACCCATGCTGAATATGATCGCTTAACGAAATACTATCGGGAGCACAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15484.61 Da        Isoelectric Point: 4.4711

>AT120932 WP_076752145.1 NZ_CP038276:c8116-7700 [Raoultella ornithinolytica]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSCERSLTLDHMRALANRFQIPVSMFVD

Download         Length: 417 bp

>AT120932 NZ_CP038276:c8116-7700 [Raoultella ornithinolytica]
ATGATTTTTAGCGATGCCATTAAAGCTGCAAATGACCTTGCCAGTATTGTACCCCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGACAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTC
TCATTGCTGCGGGTTCTTATGCAGCAACATGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTTGTGAACGCAGTCTGACCCTTGATCACATGCGAGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References