Detailed information of TA system TA120912

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF2/-
• Location: 369129..369647
• Replicon: chromosome
• Accession: NZ_CP038270
• Organism: Staphylococcus aureus strain O408 isolate B115

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: SaO408_RS01725
• Protein ID: WP_000448763.1
• Coordinates: 369345..369647 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF2
Locus tag	-
Coordinates	369129..369265 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO408_RS01685	364223..364513	+	291	WP_000179860.1	hypothetical protein	-
SaO408_RS01690	364529..366439	+	1911	WP_000429553.1	hypothetical protein	-
SaO408_RS01695	366439..367905	+	1467	WP_000067156.1	BppU family phage baseplate upper protein	-
SaO408_RS01700	367905..368294	+	390	WP_001166603.1	DUF2977 domain-containing protein	-
SaO408_RS01705	368287..368451	+	165	WP_000916020.1	XkdX family protein	-
SaO408_RS01710	368497..368796	+	300	WP_000466773.1	DUF2951 family protein	-
SaO408_RS01715	368990..369137	+	148	Protein_324	putative holin-like toxin	-
-	369129..369265	-	137	NuclAT_0	-	Antitoxin
-	369129..369265	-	137	NuclAT_0	-	Antitoxin
-	369129..369265	-	137	NuclAT_0	-	Antitoxin
-	369129..369265	-	137	NuclAT_0	-	Antitoxin
SaO408_RS01720	369184..369294	-	111	WP_078064879.1	hypothetical protein	-
SaO408_RS01725	369345..369647	+	303	WP_000448763.1	phage holin	Toxin
SaO408_RS01730	369659..371113	+	1455	WP_043039653.1	N-acetylmuramoyl-L-alanine amidase	-
SaO408_RS01740	372627..373124	+	498	WP_001803960.1	hypothetical protein	-
SaO408_RS01750	373684..374511	-	828	WP_000136010.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	328385..371113	42728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11202.94 Da
• Isoelectric Point: 9.6587

>T120912 WP_000448763.1 NZ_CP038270:369345-369647 [Staphylococcus aureus]
METKVITRYIVLILALVNQFLANKGISPIPADEESISSIILTVIALYTAYKDNPTSQEGRWANQKLKKYKAENKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T120912 NZ_CP038270:369345-369647 [Staphylococcus aureus]
ATGGAAACAAAAGTAATAACAAGATATATTGTATTAATTTTAGCGTTAGTCAATCAGTTTTTAGCGAACAAAGGTATAAG
CCCGATACCAGCAGATGAAGAGAGTATTTCATCGATTATCTTAACAGTCATTGCTTTATACACCGCTTATAAAGACAATC
CAACATCACAAGAAGGACGCTGGGCGAATCAAAAACTAAAGAAATATAAAGCTGAAAATAAGTATAGAAAAGCAACAGGA
CAAGCGCCAATTAAAGAAGTAATGACACCTACAAATATGAATGACACAAATGATTTAGGGTAG

Antitoxin

Nucleotide

• Length: 137 bp

>AT120912 NZ_CP038270:c369265-369129 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGACCTCTGTAGTTAAAT
GAATTTATATAATCCTCTAACCATCGTACTCGTCAAAGTTAGCGATGGTTATTTTTT

Structures

Antitoxin