Detailed information of TA system TA120885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 105005..105656
• Replicon: plasmid pEC_gr13
• Accession: NZ_CP038263
• Organism: Acinetobacter baumannii strain LEV1449/17Ec

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: E4K03_RS18645
• Protein ID: WP_000269904.1
• Coordinates: 105300..105656 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4K03_RS18640
• Protein ID: WP_001129974.1
• Coordinates: 105005..105307 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K03_RS18600	100426..101358	+	933	WP_005064486.1	IS5 family transposase	-
E4K03_RS18605	101558..101887	+	330	WP_004827037.1	four-helix bundle copper-binding protein	-
E4K03_RS18615	102100..103425	+	1326	WP_008942541.1	ISNCY family transposase	-
E4K03_RS18625	103559..104071	-	513	WP_001089574.1	SMI1/KNR4 family protein	-
E4K03_RS18630	104110..104628	-	519	WP_000447195.1	sel1 repeat family protein	-
E4K03_RS18635	104803..105012	-	210	WP_000069475.1	hypothetical protein	-
E4K03_RS18640	105005..105307	-	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
E4K03_RS18645	105300..105656	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4K03_RS18650	106184..106501	-	318	WP_000900860.1	hypothetical protein	-
E4K03_RS18660	106813..108138	+	1326	WP_008942541.1	ISNCY family transposase	-
E4K03_RS18670	108469..108765	+	297	WP_000569859.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4K03_RS18675	108767..109057	+	291	WP_000241337.1	putative addiction module antidote protein	-
E4K03_RS18680	109356..109508	-	153	Protein_101	transposase	-
E4K03_RS18685	109575..109736	+	162	Protein_102	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128013	128013
	-	inside	IScluster/Tn	-	-	100426..111006	10580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T120885 WP_000269904.1 NZ_CP038263:c105656-105300 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T120885 NZ_CP038263:c105656-105300 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT120885 WP_001129974.1 NZ_CP038263:c105307-105005 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT120885 NZ_CP038263:c105307-105005 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1