Detailed information of TA system TA120879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2174..2825
• Replicon: plasmid pEH_gr3
• Accession: NZ_CP038260
• Organism: Acinetobacter baumannii strain 39741

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: E4K02_RS19215
• Protein ID: WP_000269904.1
• Coordinates: 2174..2530 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4K02_RS19220
• Protein ID: WP_001129974.1
• Coordinates: 2523..2825 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K02_RS19205	448..993	+	546	WP_000736774.1	plasmid replication DNA-binding protein	-
E4K02_RS19210	1330..1647	+	318	WP_005065053.1	hypothetical protein	-
E4K02_RS19215	2174..2530	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4K02_RS19220	2523..2825	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
E4K02_RS19225	2818..3027	+	210	WP_000069475.1	hypothetical protein	-
E4K02_RS19230	3202..3720	+	519	WP_000447196.1	sel1 repeat family protein	-
E4K02_RS19235	3759..4100	+	342	Protein_7	SMI1/KNR4 family protein	-
E4K02_RS19240	4168..5333	+	1166	WP_085940844.1	IS3-like element ISAba22 family transposase	-
E4K02_RS19250	5522..7126	-	1605	WP_057073244.1	IS66 family transposase	-
E4K02_RS19255	7201..7536	-	336	WP_057073245.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25856	25856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T120879 WP_000269904.1 NZ_CP038260:2174-2530 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T120879 NZ_CP038260:2174-2530 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT120879 WP_001129974.1 NZ_CP038260:2523-2825 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT120879 NZ_CP038260:2523-2825 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1