Detailed information of TA system TA120876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 46986..47637
• Replicon: plasmid pEH_gr13
• Accession: NZ_CP038259
• Organism: Acinetobacter baumannii strain 39741

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S3SZA7
• Locus tag: E4K02_RS18670
• Protein ID: WP_000269903.1
• Coordinates: 46986..47342 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4K02_RS18675
• Protein ID: WP_001140619.1
• Coordinates: 47335..47637 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K02_RS18650	42274..43575	+	1302	WP_004704381.1	MSMEG_0569 family flavin-dependent oxidoreductase	-
E4K02_RS18655	43653..44036	+	384	WP_004704382.1	hypothetical protein	-
E4K02_RS18660	44063..44977	+	915	WP_050457731.1	metal-dependent hydrolase	-
E4K02_RS18665	45107..46432	+	1326	WP_135048405.1	ISNCY family transposase	-
E4K02_RS18670	46986..47342	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4K02_RS18675	47335..47637	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
E4K02_RS18680	47630..47839	+	210	WP_000069471.1	hypothetical protein	-
E4K02_RS18685	47949..48176	-	228	WP_025469214.1	hypothetical protein	-
E4K02_RS18690	48309..49241	-	933	WP_004739252.1	IS5-like element ISAba13 family transposase	-
E4K02_RS18695	49238..49411	-	174	WP_079270855.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4K02_RS18700	49688..50008	-	321	WP_004704395.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
E4K02_RS18705	50196..50584	+	389	Protein_51	AAA family ATPase	-
E4K02_RS18710	50512..51057	+	546	Protein_52	IS5 family transposase	-
E4K02_RS18715	51025..51279	-	255	WP_033856686.1	hypothetical protein	-
E4K02_RS18720	51341..51796	-	456	WP_004704223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135229	135229
	-	inside	IScluster/Tn	-	-	45095..58699	13604

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13486.45 Da
• Isoelectric Point: 7.1645

>T120876 WP_000269903.1 NZ_CP038259:46986-47342 [Acinetobacter baumannii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T120876 NZ_CP038259:46986-47342 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT120876 WP_001140619.1 NZ_CP038259:47335-47637 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT120876 NZ_CP038259:47335-47637 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A836MG83