Detailed information of TA system TA120871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2929286..2929884
• Replicon: chromosome
• Accession: NZ_CP038255
• Organism: Terasakiella sp. SH-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: E4K71_RS13760
• Protein ID: WP_135080542.1
• Coordinates: 2929603..2929884 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4K71_RS13755
• Protein ID: WP_135080540.1
• Coordinates: 2929286..2929594 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K71_RS13725	2924933..2925739	+	807	WP_135080530.1	peptidoglycan-binding protein	-
E4K71_RS13730	2925736..2926215	+	480	WP_135080532.1	hypothetical protein	-
E4K71_RS13735	2926458..2926769	-	312	WP_135080534.1	hypothetical protein	-
E4K71_RS13745	2928706..2928900	+	195	WP_135080536.1	hypothetical protein	-
E4K71_RS13750	2928893..2929285	+	393	WP_135080538.1	hypothetical protein	-
E4K71_RS13755	2929286..2929594	-	309	WP_135080540.1	HigA family addiction module antidote protein	Antitoxin
E4K71_RS13760	2929603..2929884	-	282	WP_135080542.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4K71_RS13765	2930063..2931211	+	1149	WP_135081968.1	IS3 family transposase	-
E4K71_RS13770	2931161..2931388	-	228	WP_135080544.1	recombinase family protein	-
E4K71_RS13775	2931750..2932013	-	264	WP_135080546.1	hypothetical protein	-
E4K71_RS13780	2932153..2932386	+	234	WP_135082079.1	type II toxin-antitoxin system ParD family antitoxin	-
E4K71_RS13785	2932379..2932669	+	291	WP_135080548.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4K71_RS13790	2932669..2933196	+	528	WP_135080549.1	hypothetical protein	-
E4K71_RS13795	2933202..2933903	-	702	WP_135080551.1	hypothetical protein	-
E4K71_RS13800	2934034..2934231	+	198	WP_135080553.1	hypothetical protein	-
E4K71_RS13805	2934273..2934533	+	261	WP_135080555.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2924606..2944118	19512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-91)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10705.40 Da
• Isoelectric Point: 10.2111

>T120871 WP_135080542.1 NZ_CP038255:c2929884-2929603 [Terasakiella sp. SH-1]
MIKSFGNKETESFFHTGKSRKLPSTILKVAMRKLDMIDAASVLEDLRVPPANRLESLSGDLSGFHSVRINKQWRIIFLWK
NDGPYDVKIVDYH

Nucleotide

• Length: 282 bp

>T120871 NZ_CP038255:c2929884-2929603 [Terasakiella sp. SH-1]
ATGATTAAGAGCTTTGGCAATAAAGAAACCGAGTCATTCTTCCATACCGGAAAAAGCCGCAAACTACCCAGTACCATCTT
AAAGGTTGCGATGCGTAAACTGGACATGATTGATGCTGCAAGTGTATTGGAGGACTTGCGTGTTCCACCAGCAAACCGTT
TAGAAAGTCTATCTGGTGATTTGTCAGGCTTTCACAGTGTTCGAATTAACAAACAATGGCGTATCATTTTTCTGTGGAAA
AACGATGGACCATACGATGTTAAAATCGTTGATTACCATTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11679.30 Da
• Isoelectric Point: 5.7472

>AT120871 WP_135080540.1 NZ_CP038255:c2929594-2929286 [Terasakiella sp. SH-1]
MSRIKTHPGEVLREEFMEPLELTAGKIAKAIDVPRDRIEKISREQKGVSADTALRLSKLFGTSPDFWMNLQSAYELSVAR
SQNNFENIHMLECLQDSETRVQ

Nucleotide

• Length: 309 bp

>AT120871 NZ_CP038255:c2929594-2929286 [Terasakiella sp. SH-1]
ATGTCACGAATTAAAACACACCCCGGTGAGGTTCTACGTGAAGAATTCATGGAACCTCTTGAACTCACTGCTGGCAAGAT
TGCCAAAGCCATTGATGTCCCACGTGACCGGATTGAGAAGATTTCACGGGAACAAAAAGGTGTCAGTGCCGATACAGCAT
TACGTTTAAGTAAGCTGTTCGGAACTTCACCAGATTTCTGGATGAATTTGCAATCTGCTTACGAGCTTTCAGTGGCGCGT
TCTCAAAACAATTTCGAGAATATTCATATGCTGGAATGCTTGCAAGATAGTGAAACACGGGTGCAATAA