Detailed information of TA system TA120831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 857429..857843
• Replicon: chromosome
• Accession: NZ_CP038229
• Organism: Staphylococcus aureus strain MJ163

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E4J95_RS04130
• Protein ID: WP_001065123.1
• Coordinates: 857429..857680 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4J95_RS04135
• Protein ID: WP_000028424.1
• Coordinates: 857670..857843 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4J95_RS04080	852966..853823	-	858	WP_000371250.1	DUF4393 domain-containing protein	-
E4J95_RS04085	853888..854658	+	771	WP_000190224.1	conserved phage C-terminal domain-containing protein	-
E4J95_RS04090	854668..855447	+	780	WP_000803065.1	ATP-binding protein	-
E4J95_RS04095	855441..855602	+	162	WP_000237153.1	hypothetical protein	-
E4J95_RS04100	855615..855836	+	222	WP_001123673.1	DUF3269 family protein	-
E4J95_RS04105	855847..856251	+	405	WP_000049784.1	DUF1064 domain-containing protein	-
E4J95_RS04110	856256..856441	+	186	WP_001187242.1	DUF3113 family protein	-
E4J95_RS04115	856442..856810	+	369	WP_001140281.1	hypothetical protein	-
E4J95_RS04120	856814..857056	+	243	WP_000131379.1	phi PVL orf 51-like protein	-
E4J95_RS04125	857071..857436	+	366	WP_001105611.1	hypothetical protein	-
E4J95_RS04130	857429..857680	+	252	WP_001065123.1	DUF1024 family protein	Toxin
E4J95_RS04135	857670..857843	+	174	WP_000028424.1	hypothetical protein	Antitoxin
E4J95_RS04140	857844..858125	+	282	WP_000454994.1	hypothetical protein	-
E4J95_RS04145	858126..858287	+	162	WP_000889683.1	hypothetical protein	-
E4J95_RS04150	858302..858835	+	534	WP_025920701.1	dUTP diphosphatase	-
E4J95_RS04155	858872..859060	+	189	WP_169449261.1	DUF1381 domain-containing protein	-
E4J95_RS04160	859035..859235	+	201	WP_001125015.1	hypothetical protein	-
E4J95_RS04165	859238..859411	+	174	WP_169449262.1	transcriptional activator RinB	-
E4J95_RS04170	859412..859558	+	147	WP_000990005.1	hypothetical protein	-
E4J95_RS04175	859582..860004	+	423	WP_031901125.1	DUF1492 domain-containing protein	-
E4J95_RS04180	860191..860631	+	441	WP_001003271.1	terminase small subunit	-
E4J95_RS04185	860618..861895	+	1278	WP_000169944.1	PBSX family phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	842012..887458	45446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9391.25 Da
• Isoelectric Point: 3.8464

>T120831 WP_001065123.1 NZ_CP038229:857429-857680 [Staphylococcus aureus]
MNNREQIEQSVISTSAYNGNDTEGLLKEIEDVYKKAQAFDEILDGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T120831 NZ_CP038229:857429-857680 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATCGAACAGTCCGTTATAAGTACTAGTGCGTATAACGGTAATGACACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGATGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT120831 WP_000028424.1 NZ_CP038229:857670-857843 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT120831 NZ_CP038229:857670-857843 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCCGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA