Detailed information of TA system TA120808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2959392..2960046
• Replicon: chromosome
• Accession: NZ_CP038207
• Organism: Pseudomonas sp. S150

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A854X939
• Locus tag: E3Z29_RS13495
• Protein ID: WP_027614658.1
• Coordinates: 2959696..2960046 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E3Z29_RS13490
• Protein ID: WP_027614657.1
• Coordinates: 2959392..2959706 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z29_RS13470	2955811..2956011	+	201	WP_134785998.1	hypothetical protein	-
E3Z29_RS13475	2956078..2956410	+	333	WP_134785999.1	DUF2790 domain-containing protein	-
E3Z29_RS13480	2956529..2957713	-	1185	WP_134786000.1	acyltransferase	-
E3Z29_RS13485	2957893..2959218	-	1326	WP_134786001.1	OprD family porin	-
E3Z29_RS13490	2959392..2959706	-	315	WP_027614657.1	transcriptional regulator	Antitoxin
E3Z29_RS13495	2959696..2960046	-	351	WP_027614658.1	toxin	Toxin
E3Z29_RS13500	2960416..2960880	+	465	WP_098968147.1	GNAT family N-acetyltransferase	-
E3Z29_RS13505	2961072..2962538	-	1467	Protein_2584	NADH-quinone oxidoreductase subunit NuoN	-
E3Z29_RS13510	2962546..2964078	-	1533	WP_003223785.1	NADH-quinone oxidoreductase subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13966.76 Da
• Isoelectric Point: 7.2031

>T120808 WP_027614658.1 NZ_CP038207:c2960046-2959696 [Pseudomonas sp. S150]
MDALFIELPVFQKHRDDYLDDDLFRSFQLELLKNPEAGDLIEDTGGLRKIRFSDQRRGKGKRSGLRVIYYWWSGFDQFWL
FTVFNKNEQDDLTPAQKKLFRKTLDRELNARSHYET

Nucleotide

• Length: 351 bp

>T120808 NZ_CP038207:c2960046-2959696 [Pseudomonas sp. S150]
ATGGATGCTCTATTTATCGAATTGCCTGTGTTTCAGAAGCACCGAGACGACTATCTGGATGACGATCTGTTTCGCAGCTT
TCAATTGGAGTTGCTGAAAAATCCGGAAGCGGGCGATCTCATCGAGGACACAGGCGGGCTTCGTAAAATTCGCTTCAGCG
ACCAACGAAGAGGGAAAGGCAAGCGCAGCGGATTACGAGTGATTTATTACTGGTGGTCAGGCTTCGATCAGTTCTGGCTC
TTTACCGTCTTCAACAAAAACGAGCAGGACGATTTGACGCCTGCCCAGAAAAAGCTTTTCAGAAAAACACTGGATAGAGA
ACTCAACGCGAGAAGCCACTATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11950.86 Da
• Isoelectric Point: 9.9896

>AT120808 WP_027614657.1 NZ_CP038207:c2959706-2959392 [Pseudomonas sp. S150]
MKRDIFSELMDGLEALADERQGKITLRTHKVQLPKLMPITAEEVIAIRQQLNLSRSVFAMYLRTNTRTLENWEQGRATPN
AQATTLIRLVERFPQTIEQLAALT

Nucleotide

• Length: 315 bp

>AT120808 NZ_CP038207:c2959706-2959392 [Pseudomonas sp. S150]
ATGAAACGTGACATTTTTTCCGAACTGATGGATGGACTGGAAGCACTGGCCGATGAACGCCAAGGCAAAATCACCTTGCG
TACCCATAAGGTTCAGCTGCCAAAACTGATGCCGATCACTGCTGAGGAAGTGATAGCCATCCGTCAGCAACTTAATCTTT
CCCGGTCAGTTTTCGCGATGTATCTACGCACCAATACGCGAACGCTCGAGAACTGGGAGCAAGGACGAGCTACGCCCAAT
GCTCAGGCCACAACCCTGATCCGTCTGGTCGAGCGTTTTCCACAGACAATCGAACAACTCGCAGCCCTGACCTGA

Structures

Toxin

• AlphaFold DB: A0A854X939