Detailed information of TA system TA120806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 854330..854934
• Replicon: chromosome
• Accession: NZ_CP038207
• Organism: Pseudomonas sp. S150

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3KJP2
• Locus tag: E3Z29_RS03945
• Protein ID: WP_011331970.1
• Coordinates: 854620..854934 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E3Z29_RS03940
• Protein ID: WP_098966690.1
• Coordinates: 854330..854617 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z29_RS03915	849460..849921	-	462	WP_007964698.1	ATP-dependent zinc protease	-
E3Z29_RS03920	850156..850563	+	408	WP_098966686.1	RNA-binding S4 domain-containing protein	-
E3Z29_RS03925	850730..851533	-	804	WP_096796730.1	phosphatase PAP2 family protein	-
E3Z29_RS03930	851639..852541	+	903	WP_098966688.1	Hsp33 family molecular chaperone HslO	-
E3Z29_RS03935	852723..854264	+	1542	WP_027611209.1	phosphoenolpyruvate carboxykinase	-
E3Z29_RS03940	854330..854617	-	288	WP_098966690.1	helix-turn-helix domain-containing protein	Antitoxin
E3Z29_RS03945	854620..854934	-	315	WP_011331970.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3Z29_RS03950	855046..855345	-	300	WP_134785191.1	hypothetical protein	-
E3Z29_RS03955	855693..856835	-	1143	WP_134785192.1	hypothetical protein	-
E3Z29_RS03960	856928..857203	-	276	WP_098966695.1	hypothetical protein	-
E3Z29_RS03965	857506..858009	+	504	WP_134785193.1	polysaccharide deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11847.76 Da
• Isoelectric Point: 10.3534

>T120806 WP_011331970.1 NZ_CP038207:c854934-854620 [Pseudomonas sp. S150]
MIFIETRIFTRRVKELLDDDTYAAFQKQLVVSPSIGDVIEGTGGIRKTRIAAKGHGKRGGARVIYYHFVSASQIGLLMIY
PKNEQHDLSSDERKALKVLIEKWR

Nucleotide

• Length: 315 bp

>T120806 NZ_CP038207:c854934-854620 [Pseudomonas sp. S150]
ATGATTTTTATTGAGACTCGGATCTTTACCCGTCGAGTAAAAGAGCTGCTGGATGATGACACGTACGCCGCTTTTCAGAA
GCAACTGGTTGTAAGCCCTTCGATTGGCGATGTAATTGAAGGCACAGGCGGGATTCGCAAAACCCGAATCGCAGCGAAAG
GACACGGCAAGCGAGGCGGAGCACGTGTTATTTACTACCACTTCGTTTCGGCGTCACAAATCGGGTTATTGATGATCTAC
CCTAAAAATGAACAACACGATCTGTCCAGCGATGAGCGTAAAGCACTCAAGGTCTTGATCGAAAAATGGAGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10777.34 Da
• Isoelectric Point: 6.8814

>AT120806 WP_098966690.1 NZ_CP038207:c854617-854330 [Pseudomonas sp. S150]
MSKFFDELMESVQEMDEIVRGERTPSREFHVDALQVKEIRKATGLTQTKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAH

Nucleotide

• Length: 288 bp

>AT120806 NZ_CP038207:c854617-854330 [Pseudomonas sp. S150]
ATGAGCAAGTTTTTTGATGAGCTGATGGAAAGTGTGCAAGAGATGGACGAGATCGTACGAGGCGAGCGCACACCTTCTCG
CGAATTCCACGTTGATGCATTGCAGGTCAAAGAAATTCGTAAAGCTACCGGTTTGACCCAGACCAAATTTGCGGCACTGA
TCGACGTCCAACTCGGCACTCTACGAAATTGGGAACAGGGCCGTCGCGAGCCAACCGGGCCGGCAAAAGCTTTGCTCAAG
GCCATTCATAACGACCCGGTTCATGTTTTGAATGCATTGGCTCATTGA

Structures

Toxin

• AlphaFold DB: Q3KJP2