Detailed information of TA system TA120800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1515395..1515978
• Replicon: chromosome
• Accession: NZ_CP038197
• Organism: Paracoccus versutus strain DSM 17099

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: E3U25_RS18735
• Protein ID: WP_116221908.1
• Coordinates: 1515697..1515978 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: E3U25_RS18730
• Protein ID: WP_116221909.1
• Coordinates: 1515395..1515685 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3U25_RS18690 (E3U25_18560)	1511390..1511761	+	372	WP_114535201.1	hypothetical protein	-
E3U25_RS18695 (E3U25_18565)	1511825..1512025	+	201	WP_116221914.1	hypothetical protein	-
E3U25_RS18700 (E3U25_18570)	1512083..1512403	-	321	WP_281029286.1	DUF983 domain-containing protein	-
E3U25_RS18705 (E3U25_18575)	1512641..1513033	-	393	WP_116221912.1	hypothetical protein	-
E3U25_RS18710 (E3U25_18580)	1513275..1513805	-	531	WP_116221911.1	hypothetical protein	-
E3U25_RS18715	1514081..1514254	-	174	WP_166435525.1	hypothetical protein	-
E3U25_RS18720	1514251..1514409	-	159	WP_166435524.1	hypothetical protein	-
E3U25_RS18725 (E3U25_18590)	1514810..1515193	+	384	WP_116221910.1	DoxX family protein	-
E3U25_RS18730 (E3U25_18595)	1515395..1515685	-	291	WP_116221909.1	HigA family addiction module antitoxin	Antitoxin
E3U25_RS18735 (E3U25_18600)	1515697..1515978	-	282	WP_116221908.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3U25_RS18740 (E3U25_18605)	1516296..1517051	-	756	WP_116220987.1	IS21-like element ISPve1 family helper ATPase IstB	-
E3U25_RS18745 (E3U25_18610)	1517048..1518502	-	1455	WP_244295116.1	IS21-like element ISPve1 family transposase	-
E3U25_RS18750 (E3U25_18615)	1518642..1519349	-	708	WP_116221907.1	hypothetical protein	-
E3U25_RS18755 (E3U25_18620)	1519511..1519831	+	321	WP_116222418.1	helix-turn-helix transcriptional regulator	-
E3U25_RS18760 (E3U25_18625)	1519828..1520748	+	921	WP_116221906.1	ImmA/IrrE family metallo-endopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1511390..1569884	58494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (16-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10255.87 Da
• Isoelectric Point: 10.5590

>T120800 WP_116221908.1 NZ_CP038197:c1515978-1515697 [Paracoccus versutus]
MIQSIRGELTAQVLAGKPGKGFPANLSKIAVRKVAMLQAAHLLDDLRAPKANRLEALKGDRAGQHSIRVNDPWRICFTWT
QNGPANVEIVDYH

Nucleotide

• Length: 282 bp

>T120800 NZ_CP038197:c1515978-1515697 [Paracoccus versutus]
ATGATACAGAGCATCAGGGGCGAGTTGACCGCGCAAGTCCTGGCTGGCAAGCCGGGCAAGGGCTTTCCCGCCAACCTGTC
CAAGATCGCCGTCCGCAAGGTGGCGATGCTGCAAGCGGCTCACCTCCTGGACGACCTGCGGGCGCCCAAGGCGAACCGGC
TGGAAGCCCTCAAGGGCGATAGGGCGGGCCAGCATTCGATCCGGGTCAACGACCCGTGGCGCATCTGTTTCACCTGGACC
CAGAACGGCCCTGCCAATGTCGAAATCGTGGACTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10690.40 Da
• Isoelectric Point: 5.5882

>AT120800 WP_116221909.1 NZ_CP038197:c1515685-1515395 [Paracoccus versutus]
MTMLQVPVHPGEILKEEFLDDLGISAGKLARHIHVPRTRIERLVKKETAMTVDTAQRLAKALGTSVEFWMNLQLNYDLIT
NTPDPDVADIQPLAAA

Nucleotide

• Length: 291 bp

>AT120800 NZ_CP038197:c1515685-1515395 [Paracoccus versutus]
ATGACCATGCTGCAAGTGCCGGTCCATCCCGGCGAAATCCTGAAGGAAGAATTCCTGGACGACTTGGGGATCAGCGCCGG
CAAGCTGGCCCGGCATATCCATGTGCCCCGCACCCGCATCGAGCGCCTTGTGAAGAAAGAAACCGCCATGACCGTGGACA
CGGCGCAGCGGCTCGCCAAGGCCCTGGGAACCTCGGTGGAGTTCTGGATGAACCTTCAACTGAACTACGACCTGATTACG
AACACGCCAGATCCAGACGTGGCCGATATCCAGCCGCTGGCAGCGGCGTGA