Detailed information of TA system TA120766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3548645..3549339
• Replicon: chromosome
• Accession: NZ_CP038180
• Organism: Escherichia coli strain

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: E4F32_RS17355
• Protein ID: WP_001263493.1
• Coordinates: 3548645..3549043 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: E4F32_RS17360
• Protein ID: WP_000554757.1
• Coordinates: 3549046..3549339 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4F32_RS17325	3543645..3544889	-	1245	WP_000189541.1	esterase FrsA	-
-	3544305..3544385	-	81	NuclAT_11	-	-
-	3544305..3544385	-	81	NuclAT_11	-	-
-	3544305..3544385	-	81	NuclAT_11	-	-
-	3544305..3544385	-	81	NuclAT_11	-	-
E4F32_RS17330	3544981..3545439	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
E4F32_RS17335	3545700..3547157	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
E4F32_RS17340	3547214..3547735	-	522	Protein_3367	peptide chain release factor H	-
E4F32_RS17345	3547734..3547937	-	204	Protein_3368	RNA ligase RtcB family protein	-
E4F32_RS17350	3548183..3548635	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
E4F32_RS17355	3548645..3549043	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
E4F32_RS17360	3549046..3549339	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
E4F32_RS17365	3549391..3550446	-	1056	WP_001226164.1	DNA polymerase IV	-
E4F32_RS17370	3550517..3551302	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
E4F32_RS17375	3551274..3552986	+	1713	Protein_3374	flagellar biosynthesis protein FlhA	-
E4F32_RS17380	3553202..3553699	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T120766 WP_001263493.1 NZ_CP038180:c3549043-3548645 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T120766 NZ_CP038180:c3549043-3548645 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT120766 WP_000554757.1 NZ_CP038180:c3549339-3549046 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT120766 NZ_CP038180:c3549339-3549046 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6