Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1793603..1794105 Replicon chromosome
Accession NZ_CP038180
Organism Escherichia coli strain

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E4F32_RS08625 Protein ID WP_000767829.1
Coordinates 1793851..1794105 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E4F32_RS08620 Protein ID WP_001259255.1
Coordinates 1793603..1793854 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4F32_RS08595 1788780..1789847 - 1068 WP_074579688.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E4F32_RS08600 1789847..1790917 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
E4F32_RS08605 1790914..1792218 - 1305 WP_087898060.1 histidinol dehydrogenase -
E4F32_RS08610 1792224..1793123 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E4F32_RS08615 1793269..1793319 - 51 WP_001364200.1 his operon leader peptide -
E4F32_RS08620 1793603..1793854 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E4F32_RS08625 1793851..1794105 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E4F32_RS08630 1794188..1795012 + 825 WP_000754737.1 SDR family oxidoreductase -
E4F32_RS08635 1795058..1795987 + 930 WP_000803366.1 LysR family transcriptional regulator -
E4F32_RS08640 1796202..1796264 + 63 WP_010723108.1 membrane protein YoeI -
E4F32_RS08645 1796254..1797612 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E4F32_RS08650 1797791..1798849 + 1059 WP_000492330.1 transport protein YeeE -
E4F32_RS08655 1798863..1799090 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T120754 WP_000767829.1 NZ_CP038180:1793851-1794105 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T120754 NZ_CP038180:1793851-1794105 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT120754 WP_001259255.1 NZ_CP038180:1793603-1793854 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT120754 NZ_CP038180:1793603-1793854 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
TCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References