Detailed information of TA system TA120723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1283960..1284614
• Replicon: chromosome
• Accession: NZ_CP038145
• Organism: Actinobacillus indolicus strain AIFJ1607

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EXH44_RS06350
• Protein ID: WP_162856720.1
• Coordinates: 1283960..1284295 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXH44_RS06355
• Protein ID: WP_162856721.1
• Coordinates: 1284306..1284614 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXH44_RS06320	1279849..1280034	+	186	WP_162856715.1	hypothetical protein	-
EXH44_RS06325	1280027..1280233	+	207	WP_162856716.1	hypothetical protein	-
EXH44_RS06330	1280235..1280543	+	309	WP_162855753.1	hypothetical protein	-
EXH44_RS06335	1280536..1280799	+	264	WP_162856717.1	hypothetical protein	-
EXH44_RS06340	1280796..1281203	+	408	WP_162856718.1	hypothetical protein	-
EXH44_RS06345	1281187..1283373	+	2187	WP_162856719.1	DUF927 domain-containing protein	-
EXH44_RS06350	1283960..1284295	+	336	WP_162856720.1	toxin	Toxin
EXH44_RS06355	1284306..1284614	+	309	WP_162856721.1	helix-turn-helix domain-containing protein	Antitoxin
EXH44_RS06360	1285426..1285740	+	315	WP_135673911.1	ribosome silencing factor	-
EXH44_RS06365	1285741..1286208	+	468	WP_135673913.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
EXH44_RS06370	1286215..1288167	+	1953	WP_162856722.1	penicillin-binding protein 2	-
EXH44_RS06375	1288160..1289287	+	1128	WP_162856723.1	rod shape-determining protein RodA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1275119..1284614	9495

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-85)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13100.14 Da
• Isoelectric Point: 10.0425

>T120723 WP_162856720.1 NZ_CP038145:1283960-1284295 [Actinobacillus indolicus]
MNLTFIELPPFERFRAENLTDEEYRAFQNELLENPEKGDVIQGLKGLRKIRIADNKRNKGKRGGARVIYYYFQTKSQIFL
IMAYGKNEQTDLTLEQQKSLITIIERIKTKL

Nucleotide

• Length: 336 bp

>T120723 NZ_CP038145:1283960-1284295 [Actinobacillus indolicus]
ATGAATTTAACTTTTATTGAATTACCACCATTCGAAAGATTTAGAGCGGAGAATTTAACAGATGAAGAGTATAGAGCTTT
CCAAAATGAATTATTAGAAAACCCTGAAAAAGGCGATGTGATACAAGGCTTAAAAGGATTGAGAAAGATAAGAATAGCAG
ACAATAAGCGGAACAAAGGCAAGCGAGGAGGAGCAAGGGTTATTTATTACTACTTTCAAACCAAAAGCCAAATATTTTTA
ATTATGGCTTACGGCAAAAATGAGCAAACCGATCTAACGTTGGAACAACAAAAATCACTAATTACAATAATTGAACGTAT
CAAAACAAAACTATAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11821.79 Da
• Isoelectric Point: 8.4924

>AT120723 WP_162856721.1 NZ_CP038145:1284306-1284614 [Actinobacillus indolicus]
MTKFYNDLMEGLTAMEEFLDGKRTLKMESLARPEPLEMRAEEIKAIREELNLSQALFALKLRTSVRTYQGWEQGKSKPNQ
QAMLLLKMVQKSPQLFEQIANA

Nucleotide

• Length: 309 bp

>AT120723 NZ_CP038145:1284306-1284614 [Actinobacillus indolicus]
ATGACAAAGTTTTATAATGATTTAATGGAAGGCTTAACAGCAATGGAGGAGTTTTTAGATGGGAAAAGAACGCTAAAAAT
GGAAAGCTTAGCACGCCCTGAGCCTTTAGAAATGCGAGCAGAAGAAATCAAAGCTATTAGAGAGGAGTTAAACCTATCAC
AAGCATTATTTGCTTTAAAATTGCGGACAAGTGTAAGAACCTATCAAGGGTGGGAGCAAGGCAAAAGCAAACCAAATCAG
CAAGCTATGCTCTTGCTTAAAATGGTACAGAAATCACCTCAACTATTTGAACAAATTGCGAATGCTTAA