Detailed information of TA system TA120717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4581680..4582282
• Replicon: chromosome
• Accession: NZ_CP038138
• Organism: Escherichia coli strain G3X16-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: E3N82_RS21960
• Protein ID: WP_000897305.1
• Coordinates: 4581971..4582282 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E3N82_RS21955
• Protein ID: WP_000356397.1
• Coordinates: 4581680..4581970 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3N82_RS21930	4577625..4578527	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
E3N82_RS21935	4578524..4579159	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
E3N82_RS21940	4579156..4580085	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
E3N82_RS21945	4580415..4580657	-	243	WP_001086388.1	hypothetical protein	-
E3N82_RS21950	4580876..4581094	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
E3N82_RS21955	4581680..4581970	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
E3N82_RS21960	4581971..4582282	-	312	WP_000897305.1	hypothetical protein	Toxin
E3N82_RS21965	4582511..4583419	+	909	WP_001162704.1	alpha/beta hydrolase	-
E3N82_RS21970	4583483..4584424	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
E3N82_RS21975	4584469..4584906	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
E3N82_RS21980	4584903..4585775	-	873	WP_000920762.1	virulence factor BrkB family protein	-
E3N82_RS21985	4585769..4586368	-	600	WP_001295269.1	glucose-1-phosphatase	-
E3N82_RS21990	4586467..4587252	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T120717 WP_000897305.1 NZ_CP038138:c4582282-4581971 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T120717 NZ_CP038138:c4582282-4581971 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT120717 WP_000356397.1 NZ_CP038138:c4581970-4581680 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT120717 NZ_CP038138:c4581970-4581680 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7