Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1878296..1878798 Replicon chromosome
Accession NZ_CP038138
Organism Escherichia coli strain G3X16-2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3N82_RS08950 Protein ID WP_000767829.1
Coordinates 1878544..1878798 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3N82_RS08945 Protein ID WP_001259255.1
Coordinates 1878296..1878547 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3N82_RS08920 1873475..1874542 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E3N82_RS08925 1874542..1875612 - 1071 WP_032151756.1 histidinol-phosphate transaminase -
E3N82_RS08930 1875609..1876913 - 1305 WP_024261505.1 histidinol dehydrogenase -
E3N82_RS08935 1876919..1877818 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E3N82_RS08940 1877964..1878014 - 51 WP_001364200.1 his operon leader peptide -
E3N82_RS08945 1878296..1878547 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3N82_RS08950 1878544..1878798 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3N82_RS08955 1878881..1879705 + 825 WP_000754749.1 SDR family oxidoreductase -
E3N82_RS08960 1879751..1880680 + 930 WP_000803351.1 LysR family transcriptional regulator -
E3N82_RS08965 1880895..1880957 + 63 WP_010723108.1 membrane protein YoeI -
E3N82_RS08970 1880947..1882305 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3N82_RS08975 1882484..1883542 + 1059 WP_032151755.1 transport protein YeeE -
E3N82_RS08980 1883556..1883783 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T120701 WP_000767829.1 NZ_CP038138:1878544-1878798 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T120701 NZ_CP038138:1878544-1878798 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT120701 WP_001259255.1 NZ_CP038138:1878296-1878547 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT120701 NZ_CP038138:1878296-1878547 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References