Detailed information of TA system TA120697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 635798..636597
• Replicon: chromosome
• Accession: NZ_CP038138
• Organism: Escherichia coli strain G3X16-2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: E3N82_RS03135
• Protein ID: WP_000347267.1
• Coordinates: 635798..636262 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: E3N82_RS03140
• Protein ID: WP_001307405.1
• Coordinates: 636262..636597 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3N82_RS03105	630799..631233	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
E3N82_RS03110	631251..632129	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
E3N82_RS03115	632119..632898	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
E3N82_RS03120	632909..633382	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
E3N82_RS03125	633405..634685	-	1281	WP_000681936.1	tagatose-bisphosphate aldolase subunit KbaZ	-
E3N82_RS03130	634934..635743	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
E3N82_RS03135	635798..636262	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
E3N82_RS03140	636262..636597	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
E3N82_RS03145	636746..638317	-	1572	WP_001273741.1	galactarate dehydratase	-
E3N82_RS03150	638692..640026	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
E3N82_RS03155	640042..640812	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T120697 WP_000347267.1 NZ_CP038138:c636262-635798 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T120697 NZ_CP038138:c636262-635798 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT120697 WP_001307405.1 NZ_CP038138:c636597-636262 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT120697 NZ_CP038138:c636597-636262 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7