Detailed information of TA system TA120687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3609408..3609983
• Replicon: chromosome
• Accession: NZ_CP038033
• Organism: Nitrosococcus wardiae strain D1FHS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E3U44_RS16980
• Protein ID: WP_134359266.1
• Coordinates: 3609408..3609686 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E3U44_RS16985
• Protein ID: WP_134359901.1
• Coordinates: 3609696..3609983 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3U44_RS16930	3604501..3605097	+	597	WP_134359260.1	hypothetical protein	-
E3U44_RS16935	3605084..3605524	+	441	WP_134359261.1	hypothetical protein	-
E3U44_RS16940	3605521..3606102	+	582	WP_134359262.1	hypothetical protein	-
E3U44_RS16945	3606244..3607077	+	834	WP_134359263.1	ion transporter	-
E3U44_RS16950	3607356..3607559	-	204	Protein_3359	type II toxin-antitoxin system RelE/ParE family toxin	-
E3U44_RS16955	3607543..3607767	-	225	WP_013033471.1	TraY domain-containing protein	-
E3U44_RS16970	3608528..3608797	-	270	WP_134359264.1	Txe/YoeB family addiction module toxin	-
E3U44_RS16975	3608778..3609050	-	273	WP_134359265.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
E3U44_RS16980	3609408..3609686	+	279	WP_134359266.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3U44_RS16985	3609696..3609983	+	288	WP_134359901.1	HigA family addiction module antidote protein	Antitoxin
E3U44_RS16990	3610054..3610899	-	846	WP_134359267.1	helix-turn-helix domain-containing protein	-
E3U44_RS16995	3610889..3611287	-	399	WP_134359268.1	IS607 family transposase	-
E3U44_RS17000	3611629..3611874	-	246	WP_134359269.1	hypothetical protein	-
E3U44_RS17005	3612107..3612637	+	531	WP_134359270.1	YchJ family protein	-
E3U44_RS17010	3612782..3613156	-	375	WP_134359271.1	YajD family HNH nuclease	-
E3U44_RS17015	3613451..3613726	+	276	WP_134359272.1	hypothetical protein	-
E3U44_RS17020	3613740..3614195	+	456	WP_134359273.1	hypothetical protein	-
E3U44_RS17025	3614664..3614915	+	252	WP_134359274.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10675.31 Da
• Isoelectric Point: 10.9304

>T120687 WP_134359266.1 NZ_CP038033:3609408-3609686 [Nitrosococcus wardiae]
MIRSFKHKGLERFFLKGTKSGIQAKHAVRLRLILGRLNAATNPQDMNLPGLKLHELSGRRKGFWSAWVSGNWRVTFRFEG
MDAEAIDYEDYH

Nucleotide

• Length: 279 bp

>T120687 NZ_CP038033:3609408-3609686 [Nitrosococcus wardiae]
ATGATACGTAGCTTCAAGCACAAAGGCCTTGAGAGGTTCTTCCTGAAAGGAACCAAGTCAGGAATCCAGGCTAAACATGC
CGTCCGATTGAGGCTGATACTCGGCCGGCTGAATGCTGCTACTAACCCCCAGGACATGAACCTTCCTGGGCTCAAACTAC
ATGAGCTAAGTGGTCGGCGTAAAGGATTCTGGTCAGCTTGGGTTAGTGGTAACTGGCGTGTGACATTTCGTTTTGAGGGA
ATGGATGCTGAAGCTATTGATTATGAAGATTATCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10489.11 Da
• Isoelectric Point: 9.1032

>AT120687 WP_134359901.1 NZ_CP038033:3609696-3609983 [Nitrosococcus wardiae]
MKMHNPPHPGEVLRELCIEPMGLSITETAEALGVSRKTLSAILNGHAGISPEMAVRLSLAFGTSSESWLNQQSQYDLWEA
EKKRKSLKVRKLSAT

Nucleotide

• Length: 288 bp

>AT120687 NZ_CP038033:3609696-3609983 [Nitrosococcus wardiae]
ATGAAGATGCACAACCCACCCCATCCCGGTGAAGTACTCAGGGAACTATGCATCGAGCCAATGGGGTTGAGTATTACTGA
GACTGCCGAAGCGCTTGGTGTTAGCAGGAAGACTTTGTCTGCTATCTTGAATGGCCATGCAGGCATTAGTCCTGAAATGG
CGGTTCGTCTTTCTTTGGCATTTGGAACGTCTTCCGAGAGCTGGCTGAATCAGCAATCCCAATATGATCTATGGGAAGCC
GAGAAAAAGCGCAAGTCACTAAAAGTGAGGAAGTTGTCAGCAACCTAA