Detailed information of TA system TA120679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 16489..17176
• Replicon: plasmid unnamed2
• Accession: NZ_CP038032
• Organism: Rhodococcus ruber strain R1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E2561_RS25110
• Protein ID: WP_140189311.1
• Coordinates: 16805..17176 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E2561_RS25105
• Protein ID: WP_140189310.1
• Coordinates: 16489..16803 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2561_RS25065	11960..12406	+	447	WP_140189303.1	hypothetical protein	-
E2561_RS25070	12779..12985	+	207	WP_140189304.1	hypothetical protein	-
E2561_RS25075	13059..13310	-	252	WP_140189305.1	hypothetical protein	-
E2561_RS25080	13277..13534	-	258	WP_140189306.1	hypothetical protein	-
E2561_RS25085	13890..14723	+	834	WP_140189319.1	replication initiation protein	-
E2561_RS25090	14723..15013	+	291	WP_140189307.1	helix-turn-helix domain-containing protein	-
E2561_RS25095	15334..15579	-	246	WP_140189308.1	hypothetical protein	-
E2561_RS25100	15576..16163	-	588	WP_140189309.1	ParA family protein	-
E2561_RS25105	16489..16803	-	315	WP_140189310.1	helix-turn-helix transcriptional regulator	Antitoxin
E2561_RS25110	16805..17176	-	372	WP_140189311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E2561_RS25115	17583..18884	+	1302	WP_140189312.1	hypothetical protein	-
E2561_RS25120	19999..20181	+	183	WP_140189313.1	hypothetical protein	-
E2561_RS25125	20341..20529	-	189	WP_140189314.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33016	33016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (21-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13928.78 Da
• Isoelectric Point: 4.7552

>T120679 WP_140189311.1 NZ_CP038032:c17176-16805 [Rhodococcus ruber]
MAWEVVLLDEVDEWYLRLVRTDAETATAVTAAIDSLESEGPTLGRPLVDKVKGSHVHNMKELRPSGTSVRILFVFDPRRQ
AVLLVAGDKAGAWKQWYVDNIPRAEERYTMWLTEQNTSGTQEV

Nucleotide

• Length: 372 bp

>T120679 NZ_CP038032:c17176-16805 [Rhodococcus ruber]
ATGGCCTGGGAGGTCGTACTGCTCGACGAGGTCGATGAGTGGTATCTGCGTCTCGTGCGGACCGATGCCGAGACGGCGAC
GGCCGTCACCGCGGCCATCGACAGCTTGGAAAGCGAAGGGCCCACCTTGGGACGTCCCCTCGTGGACAAGGTCAAAGGCT
CGCACGTGCACAACATGAAAGAACTCCGTCCCAGCGGCACCTCGGTACGCATCCTCTTCGTCTTCGACCCCCGGCGGCAG
GCGGTACTGCTGGTCGCCGGAGACAAGGCCGGCGCCTGGAAGCAGTGGTACGTCGACAACATCCCGAGAGCCGAGGAGCG
CTACACGATGTGGCTCACAGAGCAGAACACCAGCGGTACCCAGGAGGTGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11964.42 Da
• Isoelectric Point: 6.7327

>AT120679 WP_140189310.1 NZ_CP038032:c16803-16489 [Rhodococcus ruber]
MARHWREVRADAAERLDEQAVTEARKSMQEEVRAYRLAEIRREQGWTRQEDVADLMGISQSRVSKIERGDLRHTELGTLE
SYVRALGGHLRVVAEFGDRSLTLG

Nucleotide

• Length: 315 bp

>AT120679 NZ_CP038032:c16803-16489 [Rhodococcus ruber]
ATGGCGCGACATTGGCGCGAAGTGCGGGCGGACGCCGCCGAGCGGCTCGACGAGCAGGCCGTCACCGAGGCCCGCAAGAG
CATGCAGGAAGAGGTGAGGGCGTATCGCCTCGCCGAGATCCGCCGCGAACAGGGCTGGACCCGGCAGGAGGACGTGGCCG
ACCTGATGGGAATCTCCCAGTCCCGGGTCTCGAAGATCGAACGTGGCGACCTGCGACACACAGAACTCGGCACCCTGGAG
TCCTACGTGCGGGCCCTCGGCGGGCACCTCCGTGTCGTCGCGGAATTCGGTGACCGCTCCCTAACCCTGGGATAG