Detailed information of TA system TA120624

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3431212..3431437
• Replicon: chromosome
• Accession: NZ_CP037996
• Organism: Shigella flexneri strain FC906

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CBL25_RS17730
• Protein ID: WP_000813254.1
• Coordinates: 3431212..3431367 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3431379..3431437 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBL25_RS17670	3426615..3426965	+	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-
CBL25_RS24610	3426962..3427255	+	294	WP_252997104.1	transposase	-
CBL25_RS24615	3427404..3427484	+	81	WP_252997118.1	hypothetical protein	-
CBL25_RS17680	3427513..3428210	+	698	WP_223368647.1	IS1 family transposase	-
CBL25_RS17705	3428654..3429343	-	690	WP_134799254.1	bacteriophage antitermination protein Q	-
CBL25_RS17710	3429340..3429705	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
CBL25_RS17715	3429706..3430764	-	1059	WP_134799255.1	DUF968 domain-containing protein	-
CBL25_RS17720	3430766..3431044	-	279	WP_011069426.1	hypothetical protein	-
CBL25_RS17730	3431212..3431367	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3431379..3431437	+	59	-	-	Antitoxin
CBL25_RS17745	3432018..3432434	-	417	WP_005069274.1	hypothetical protein	-
CBL25_RS17750	3432461..3432601	+	141	Protein_3398	DUF4224 domain-containing protein	-
CBL25_RS17755	3432601..3433648	+	1048	Protein_3399	tyrosine-type recombinase/integrase	-
CBL25_RS17765	3433859..3434656	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
CBL25_RS17775	3434994..3436256	+	1263	Protein_3401	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	3405520..3439020	33500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T120624 WP_000813254.1 NZ_CP037996:c3431367-3431212 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T120624 NZ_CP037996:c3431367-3431212 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT120624 NZ_CP037996:3431379-3431437 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin