Detailed information of TA system TA120613

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2760244..2760469
• Replicon: chromosome
• Accession: NZ_CP037996
• Organism: Shigella flexneri strain FC906

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CBL25_RS13925
• Protein ID: WP_000813254.1
• Coordinates: 2760314..2760469 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2760244..2760302 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBL25_RS24475	2756247..2756416	+	170	Protein_2662	hypothetical protein	-
CBL25_RS13890	2756562..2757305	+	744	WP_000788999.1	ATP-binding protein	-
CBL25_RS13895	2757320..2757742	+	423	WP_001118168.1	DUF977 family protein	-
CBL25_RS13900	2757799..2758155	+	357	WP_005048249.1	hypothetical protein	-
CBL25_RS13905	2758217..2758465	+	249	WP_134799303.1	DUF4014 family protein	-
CBL25_RS13910	2758467..2758832	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
CBL25_RS13915	2758829..2759494	+	666	WP_000208062.1	hypothetical protein	-
CBL25_RS13920	2759494..2759859	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	2760244..2760302	-	59	-	-	Antitoxin
CBL25_RS13925	2760314..2760469	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
CBL25_RS13940	2761806..2762405	+	600	WP_000940329.1	DUF1367 family protein	-
CBL25_RS13945	2762405..2762695	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
CBL25_RS13950	2762692..2763138	+	447	Protein_2674	DUF1133 family protein	-
CBL25_RS13955	2763147..2764312	-	1166	Protein_2675	IS3 family transposase	-
CBL25_RS13960	2764385..2764495	+	111	Protein_2676	DUF1133 family protein	-
CBL25_RS13965	2764648..2764821	+	174	WP_000504450.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	2752341..2816673	64332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T120613 WP_000813254.1 NZ_CP037996:2760314-2760469 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T120613 NZ_CP037996:2760314-2760469 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT120613 NZ_CP037996:c2760302-2760244 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin