Detailed information of TA system TA120609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1705768..1706612
• Replicon: chromosome
• Accession: NZ_CP037996
• Organism: Shigella flexneri strain FC906

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: CBL25_RS08385
• Protein ID: WP_014532172.1
• Coordinates: 1705768..1706229 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: CBL25_RS08395
• Protein ID: WP_005053053.1
• Coordinates: 1706301..1706612 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBL25_RS08370 (1700936)	1700936..1702666	-	1731	WP_000645013.1	hypothetical protein	-
CBL25_RS08375 (1702719)	1702719..1704872	-	2154	WP_005060811.1	SEL1-like repeat protein	-
CBL25_RS08380 (1705023)	1705023..1705720	+	698	WP_252965753.1	IS1 family transposase	-
CBL25_RS08385 (1705768)	1705768..1706229	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
CBL25_RS08395 (1706301)	1706301..1706612	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
CBL25_RS08400 (1706797)	1706797..1707687	-	891	WP_000971346.1	heme o synthase	-
CBL25_RS08405 (1707699)	1707699..1708028	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CBL25_RS08410 (1708028)	1708028..1708642	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CBL25_RS08415 (1708632)	1708632..1710623	-	1992	Protein_1618	cytochrome o ubiquinol oxidase subunit I	-
CBL25_RS08420 (1710645)	1710645..1711142	-	498	Protein_1619	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.007	flank	IS/Tn	-	-	1705217..1705720	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T120609 WP_014532172.1 NZ_CP037996:c1706229-1705768 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T120609 NZ_CP037996:c1706229-1705768 [Shigella flexneri]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT120609 WP_005053053.1 NZ_CP037996:c1706612-1706301 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT120609 NZ_CP037996:c1706612-1706301 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0