120608

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1624507..1625201 Replicon chromosome
Accession NZ_CP037996
Organism Shigella flexneri strain FC906

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag CBL25_RS07965 Protein ID WP_001263491.1
Coordinates 1624803..1625201 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag CBL25_RS07960 Protein ID WP_000554759.1
Coordinates 1624507..1624800 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CBL25_RS07940 (1620131) 1620131..1620628 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
CBL25_RS07945 (1620860) 1620860..1622572 - 1713 Protein_1528 flagellar biosynthesis protein FlhA -
CBL25_RS07950 (1622544) 1622544..1623329 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
CBL25_RS07955 (1623400) 1623400..1624455 + 1056 WP_001226172.1 DNA polymerase IV -
CBL25_RS07960 (1624507) 1624507..1624800 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CBL25_RS07965 (1624803) 1624803..1625201 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CBL25_RS07970 (1625211) 1625211..1625663 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
CBL25_RS07975 (1625908) 1625908..1626951 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
CBL25_RS07980 (1627067) 1627067..1627627 + 561 Protein_1535 peptide chain release factor H -
CBL25_RS07985 (1627684) 1627684..1629141 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
CBL25_RS07990 (1629402) 1629402..1629860 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T120608 WP_001263491.1 NZ_CP037996:1624803-1625201 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T120608 NZ_CP037996:1624803-1625201 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT120608 WP_000554759.1 NZ_CP037996:1624507-1624800 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT120608 NZ_CP037996:1624507-1624800 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References