Detailed information of TA system TA120599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2740316..2740889
• Replicon: chromosome
• Accession: NZ_CP037993
• Organism: Herbaspirillum huttiense strain NFYY 53159

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7X2LN43
• Locus tag: E2K99_RS12425
• Protein ID: WP_034302356.1
• Coordinates: 2740611..2740889 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E2K99_RS12420
• Protein ID: WP_034302315.1
• Coordinates: 2740316..2740591 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2K99_RS12395	2736055..2736630	+	576	WP_134222393.1	recombinase family protein	-
E2K99_RS12400	2736635..2738269	+	1635	WP_134224091.1	DDE-type integrase/transposase/recombinase	-
E2K99_RS12405	2738259..2739230	+	972	WP_134222394.1	ExeA family protein	-
E2K99_RS12410	2739239..2739943	+	705	WP_134222395.1	hypothetical protein	-
E2K99_RS12415	2740035..2740229	-	195	WP_134222396.1	hypothetical protein	-
E2K99_RS12420	2740316..2740591	-	276	WP_034302315.1	HigA family addiction module antidote protein	Antitoxin
E2K99_RS12425	2740611..2740889	-	279	WP_034302356.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E2K99_RS12430	2741165..2741875	-	711	WP_034302359.1	hypothetical protein	-
E2K99_RS12435	2742011..2744950	-	2940	WP_034302318.1	Tn3 family transposase	-
E2K99_RS25510	2745040..2745186	+	147	WP_166675062.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2723839..2748817	24978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-79)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10248.75 Da
• Isoelectric Point: 8.6443

>T120599 WP_034302356.1 NZ_CP037993:c2740889-2740611 [Herbaspirillum huttiense]
MIKSFRHKGLHRFFETGSKAGIQAAHAAKLRLQLAALDQAVQPEDLSAPSWGLHPLKGDLKGHWAITVNGNWRLIFAFEG
ADAILVDYLDYH

Nucleotide

• Length: 279 bp

>T120599 NZ_CP037993:c2740889-2740611 [Herbaspirillum huttiense]
ATGATTAAAAGCTTTCGACACAAAGGGCTGCACCGCTTCTTTGAAACGGGTAGCAAAGCTGGAATCCAGGCCGCACACGC
GGCCAAATTGCGGCTTCAACTGGCAGCACTGGATCAAGCTGTACAGCCGGAAGATTTATCAGCCCCATCGTGGGGACTGC
ATCCTTTGAAAGGTGATCTAAAGGGACATTGGGCAATTACGGTCAATGGAAACTGGCGATTGATTTTCGCTTTTGAGGGT
GCGGACGCGATCCTTGTGGATTATCTCGACTATCACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9909.45 Da
• Isoelectric Point: 10.0899

>AT120599 WP_034302315.1 NZ_CP037993:c2740591-2740316 [Herbaspirillum huttiense]
MTRMHNPPHPGEVLREYLGDVTVTEAATRLGVGRVTLQRVVTGAAGISPDMAYRLGSAFGTSPELWAGMQLQFDLYQAGK
VKRPKIERIAA

Nucleotide

• Length: 276 bp

>AT120599 NZ_CP037993:c2740591-2740316 [Herbaspirillum huttiense]
ATGACTCGCATGCATAACCCGCCTCACCCCGGAGAGGTGTTGAGAGAATATCTGGGGGATGTCACCGTGACTGAGGCCGC
CACTCGTCTTGGCGTTGGCCGGGTCACGCTTCAACGTGTCGTTACGGGCGCGGCCGGTATTTCGCCTGACATGGCGTACC
GCCTTGGTTCTGCCTTCGGTACCAGCCCTGAATTGTGGGCAGGAATGCAGCTGCAATTCGACTTATACCAGGCCGGCAAG
GTCAAGCGCCCAAAAATTGAACGCATCGCAGCATAG

Structures

Toxin

• AlphaFold DB: A0A7X2LN43