Detailed information of TA system TA120557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3675518..3676118
• Replicon: chromosome
• Accession: NZ_CP037952
• Organism: Parashewanella spongiae strain HJ039

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E2I05_RS14520
• Protein ID: WP_121851680.1
• Coordinates: 3675828..3676118 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E2I05_RS14515
• Protein ID: WP_121851627.1
• Coordinates: 3675518..3675820 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2I05_RS14480	3670577..3671743	+	1167	WP_133309712.1	flagella assembly protein FlgT	-
E2I05_RS14490	3672016..3672657	-	642	WP_121851632.1	LexA family transcriptional regulator	-
E2I05_RS14495	3672838..3673689	+	852	WP_121851631.1	hypothetical protein	-
E2I05_RS14500	3673691..3674287	+	597	WP_121851630.1	hypothetical protein	-
E2I05_RS14505	3674284..3674778	+	495	WP_121851629.1	hypothetical protein	-
E2I05_RS14510	3674784..3675287	-	504	WP_121851628.1	hypothetical protein	-
E2I05_RS14515	3675518..3675820	-	303	WP_121851627.1	HigA family addiction module antidote protein	Antitoxin
E2I05_RS14520	3675828..3676118	-	291	WP_121851680.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E2I05_RS14525	3676295..3677008	-	714	WP_121851626.1	queuosine precursor transporter	-
E2I05_RS14530	3676987..3677445	-	459	WP_133309713.1	hypothetical protein	-
E2I05_RS14535	3677445..3678155	-	711	WP_121851624.1	class I SAM-dependent methyltransferase	-
E2I05_RS14540	3678532..3679083	+	552	WP_145964474.1	plasmid mobilization relaxosome protein MobC	-
E2I05_RS14545	3679064..3679723	+	660	WP_121851679.1	relaxase/mobilization nuclease domain-containing protein	-
E2I05_RS14550	3679737..3680399	-	663	WP_121851622.1	hypothetical protein	-
E2I05_RS14555	3680482..3680904	-	423	WP_121851621.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-68)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11720.51 Da
• Isoelectric Point: 7.3194

>T120557 WP_121851680.1 NZ_CP037952:c3676118-3675828 [Parashewanella spongiae]
MIITFKHSWLENFYEEDKSHKKIPNELEDRLYGKLQLLDAAKMECDLRLPPSNKFEHLKGNLKEYCSIRVNKQYRLIFKW
IEGNVEDLYLDKHEYK

Nucleotide

• Length: 291 bp

>T120557 NZ_CP037952:c3676118-3675828 [Parashewanella spongiae]
ATGATTATTACCTTTAAACATTCATGGCTTGAGAACTTCTATGAAGAAGACAAAAGCCATAAAAAGATCCCAAATGAGCT
TGAGGATAGGTTATATGGAAAGCTTCAATTACTCGATGCCGCAAAAATGGAGTGCGACTTAAGGCTTCCACCCTCAAATA
AGTTTGAGCATTTAAAAGGGAACCTGAAAGAGTATTGCTCAATAAGAGTAAACAAACAGTATAGGCTTATTTTCAAGTGG
ATTGAAGGCAATGTCGAAGACCTTTATTTAGATAAGCACGAATACAAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11191.92 Da
• Isoelectric Point: 9.0366

>AT120557 WP_121851627.1 NZ_CP037952:c3675820-3675518 [Parashewanella spongiae]
MRKTKRKPLTVGQMLQIEFMKPYGLTNQQLADAMGVHRNTVSNIVNGGILTAPNAIKLACALGNTPEFWLNIQHAVDLWE
TRNSYESESKSVHPLELFPA

Nucleotide

• Length: 303 bp

>AT120557 NZ_CP037952:c3675820-3675518 [Parashewanella spongiae]
ATGCGTAAGACAAAGCGCAAACCACTAACAGTGGGTCAAATGCTACAGATTGAGTTTATGAAGCCATATGGCTTAACCAA
TCAACAATTAGCTGATGCGATGGGAGTCCACCGAAATACTGTTAGCAATATTGTTAATGGTGGAATATTAACAGCACCAA
ATGCAATAAAACTTGCTTGTGCTCTAGGGAATACTCCTGAGTTTTGGCTTAATATTCAGCATGCTGTTGATCTATGGGAA
ACTAGAAACAGCTATGAAAGTGAATCAAAGAGTGTTCACCCATTAGAGCTTTTTCCGGCATAG