Detailed information of TA system TA120532

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30797..31067
• Replicon: plasmid pCFSAN027346-2
• Accession: NZ_CP037947
• Organism: Escherichia coli strain CFSAN027346

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EKI52_RS29775
• Protein ID: WP_001312861.1
• Coordinates: 30909..31067 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30797..30860 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI52_RS29730	25804..26118	+	315	WP_133342201.1	hypothetical protein	-
EKI52_RS29745	26591..27118	+	528	WP_000290816.1	single-stranded DNA-binding protein	-
EKI52_RS29750	27174..27407	+	234	WP_058653442.1	DUF905 domain-containing protein	-
EKI52_RS29755	27466..29424	+	1959	WP_058653444.1	ParB/RepB/Spo0J family partition protein	-
EKI52_RS29760	29479..29913	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
EKI52_RS29765	29910..30629	+	720	WP_033807739.1	plasmid SOS inhibition protein A	-
-	30641..30865	+	225	NuclAT_0	-	-
-	30641..30865	+	225	NuclAT_0	-	-
-	30641..30865	+	225	NuclAT_0	-	-
-	30641..30865	+	225	NuclAT_0	-	-
-	30797..30860	-	64	-	-	Antitoxin
EKI52_RS29775	30909..31067	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
EKI52_RS29780	31305..31682	-	378	Protein_45	hypothetical protein	-
EKI52_RS29790	31982..32278	+	297	WP_001272251.1	hypothetical protein	-
EKI52_RS29795	32389..33210	+	822	WP_021503380.1	DUF945 domain-containing protein	-
EKI52_RS29800	33506..34153	-	648	WP_072208664.1	transglycosylase SLT domain-containing protein	-
EKI52_RS29805	34439..34822	+	384	WP_001151564.1	relaxosome protein TraM	-
EKI52_RS29810	35016..35702	+	687	WP_000332487.1	PAS domain-containing protein	-
EKI52_RS29815	35796..36023	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / dfrA8 / tet(B)	-	1..73152	73152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T120532 WP_001312861.1 NZ_CP037947:30909-31067 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T120532 NZ_CP037947:30909-31067 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT120532 NZ_CP037947:c30860-30797 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin