Detailed information of TA system TA120525

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 5225773..5226144
• Replicon: chromosome
• Accession: NZ_CP037945
• Organism: Escherichia coli strain CFSAN027346

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: EKI52_RS26905
• Protein ID: WP_042853000.1
• Coordinates: 5225950..5226144 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	5225773..5225951 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI52_RS26865	5221216..5221872	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
EKI52_RS26870	5221974..5222204	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
EKI52_RS26875	5222343..5222717	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
EKI52_RS26880	5222721..5223593	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
EKI52_RS26885	5223606..5223947	+	342	WP_000976492.1	YebY family protein	-
EKI52_RS26890	5224300..5225415	-	1116	WP_001189092.1	phage integrase Arm DNA-binding domain-containing protein	-
EKI52_RS26895	5225381..5225662	-	282	WP_001443927.1	excisionase	-
EKI52_RS26900	5225769..5225957	-	189	WP_001356607.1	DUF1187 family protein	-
-	5225773..5225951	+	179	NuclAT_1	-	Antitoxin
-	5225773..5225951	+	179	NuclAT_1	-	Antitoxin
-	5225773..5225951	+	179	NuclAT_1	-	Antitoxin
-	5225773..5225951	+	179	NuclAT_1	-	Antitoxin
EKI52_RS26905	5225950..5226144	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EKI52_RS26910	5226201..5227010	-	810	WP_000166317.1	recombination protein RecT	-
EKI52_RS26915	5227003..5229654	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
EKI52_RS26920	5229753..5230028	-	276	WP_001307773.1	hypothetical protein	-
EKI52_RS26925	5230102..5230278	-	177	WP_000245534.1	hypothetical protein	-
EKI52_RS26930	5230272..5230493	-	222	WP_000560218.1	killing protein KilR	-
EKI52_RS26935	5230914..5231066	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	5216565..5289464	72899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T120525 WP_042853000.1 NZ_CP037945:c5226144-5225950 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T120525 NZ_CP037945:c5226144-5225950 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT120525 NZ_CP037945:5225773-5225951 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin