Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4960276..4960778 Replicon chromosome
Accession NZ_CP037945
Organism Escherichia coli strain CFSAN027346

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EKI52_RS25480 Protein ID WP_000767829.1
Coordinates 4960524..4960778 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EKI52_RS25475 Protein ID WP_001259255.1
Coordinates 4960276..4960527 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI52_RS25450 4955457..4956524 - 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EKI52_RS25455 4956524..4957594 - 1071 WP_000108914.1 histidinol-phosphate transaminase -
EKI52_RS25460 4957591..4958895 - 1305 WP_000009589.1 histidinol dehydrogenase -
EKI52_RS25465 4958901..4959800 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EKI52_RS25470 4959946..4959993 - 48 WP_001375657.1 his operon leader peptide -
EKI52_RS25475 4960276..4960527 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EKI52_RS25480 4960524..4960778 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EKI52_RS25485 4960861..4961685 + 825 WP_000754737.1 SDR family oxidoreductase -
EKI52_RS25490 4961731..4962660 + 930 WP_000803351.1 LysR family transcriptional regulator -
EKI52_RS25495 4962875..4962937 + 63 WP_010723108.1 membrane protein YoeI -
EKI52_RS25500 4962927..4964285 + 1359 WP_096955198.1 putrescine/proton symporter PlaP -
EKI52_RS25505 4964464..4965522 + 1059 WP_000492324.1 transport protein YeeE -
EKI52_RS25510 4965536..4965763 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T120515 WP_000767829.1 NZ_CP037945:4960524-4960778 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T120515 NZ_CP037945:4960524-4960778 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT120515 WP_001259255.1 NZ_CP037945:4960276-4960527 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT120515 NZ_CP037945:4960276-4960527 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References