Detailed information of TA system TA120513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4078067..4078794
• Replicon: chromosome
• Accession: NZ_CP037945
• Organism: Escherichia coli strain CFSAN027346

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: EKI52_RS20945
• Protein ID: WP_000547559.1
• Coordinates: 4078067..4078378 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EKI52_RS20950
• Protein ID: WP_000126294.1
• Coordinates: 4078375..4078794 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI52_RS20915	4073209..4074918	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
EKI52_RS20920	4074928..4075470	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
EKI52_RS20925	4075470..4076237	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
EKI52_RS20930	4076234..4076644	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
EKI52_RS20935	4076637..4077107	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EKI52_RS20940	4077150..4077893	+	744	WP_001341829.1	hypothetical protein	-
EKI52_RS20945	4078067..4078378	+	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
EKI52_RS20950	4078375..4078794	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EKI52_RS20955	4078908..4080332	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
EKI52_RS20960	4080341..4081798	-	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EKI52_RS20965	4082058..4083068	+	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
EKI52_RS20970	4083217..4083744	+	528	WP_001078781.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T120513 WP_000547559.1 NZ_CP037945:4078067-4078378 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T120513 NZ_CP037945:4078067-4078378 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT120513 WP_000126294.1 NZ_CP037945:4078375-4078794 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT120513 NZ_CP037945:4078375-4078794 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163