Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4366567..4367102 Replicon chromosome
Accession NZ_CP037943
Organism Escherichia coli strain CFSAN027343

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag EKI57_RS22320 Protein ID WP_032183258.1
Coordinates 4366567..4366854 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EKI57_RS22325 Protein ID WP_000729704.1
Coordinates 4366842..4367102 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI57_RS22305 4364149..4364727 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EKI57_RS22310 4364933..4365700 + 768 WP_000333380.1 class II glutamine amidotransferase -
EKI57_RS22315 4365671..4366411 - 741 WP_001225679.1 murein L,D-transpeptidase -
EKI57_RS22320 4366567..4366854 - 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EKI57_RS22325 4366842..4367102 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EKI57_RS22330 4367288..4368061 + 774 WP_000543897.1 C40 family peptidase -
EKI57_RS22335 4368118..4368474 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EKI57_RS22340 4368467..4368745 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EKI57_RS22345 4368850..4370943 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
EKI57_RS22350 4370927..4372066 - 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T120493 WP_032183258.1 NZ_CP037943:c4366854-4366567 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T120493 NZ_CP037943:c4366854-4366567 [Escherichia coli]
ATTAGGAATTTAAATCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAA
GGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACC
CGCTGCAAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAA
CTTTTACGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT120493 WP_000729704.1 NZ_CP037943:c4367102-4366842 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT120493 NZ_CP037943:c4367102-4366842 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References