Detailed information of TA system TA120487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3439408..3440010
• Replicon: chromosome
• Accession: NZ_CP037943
• Organism: Escherichia coli strain CFSAN027343

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EKI57_RS17860
• Protein ID: WP_000897305.1
• Coordinates: 3439408..3439719 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EKI57_RS17865
• Protein ID: WP_000356397.1
• Coordinates: 3439720..3440010 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI57_RS17830	3434438..3435223	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
EKI57_RS17835	3435322..3435921	+	600	WP_001315111.1	glucose-1-phosphatase	-
EKI57_RS17840	3435915..3436787	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EKI57_RS17845	3436784..3437221	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EKI57_RS17850	3437266..3438207	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EKI57_RS17855	3438271..3439179	-	909	WP_001162704.1	alpha/beta hydrolase	-
EKI57_RS17860	3439408..3439719	+	312	WP_000897305.1	hypothetical protein	Toxin
EKI57_RS17865	3439720..3440010	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EKI57_RS17870	3440615..3440833	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EKI57_RS17875	3441052..3441294	+	243	WP_001086390.1	hypothetical protein	-
EKI57_RS17880	3441523..3442503	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
EKI57_RS17890	3442903..3443832	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EKI57_RS17895	3443829..3444464	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3441523..3442503	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T120487 WP_000897305.1 NZ_CP037943:3439408-3439719 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T120487 NZ_CP037943:3439408-3439719 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT120487 WP_000356397.1 NZ_CP037943:3439720-3440010 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT120487 NZ_CP037943:3439720-3440010 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7