120481

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1244876..1245378 Replicon chromosome
Accession NZ_CP037943
Organism Escherichia coli strain CFSAN027343

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EKI57_RS06895 Protein ID WP_000767829.1
Coordinates 1244876..1245130 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EKI57_RS06900 Protein ID WP_001259255.1
Coordinates 1245127..1245378 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI57_RS06865 1239891..1240118 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EKI57_RS06870 1240132..1241190 - 1059 WP_000492324.1 transport protein YeeE -
EKI57_RS06875 1241369..1242727 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EKI57_RS06880 1242717..1242779 - 63 WP_010723108.1 membrane protein YoeI -
EKI57_RS06885 1242994..1243923 - 930 WP_000803351.1 LysR family transcriptional regulator -
EKI57_RS06890 1243969..1244793 - 825 WP_000754737.1 SDR family oxidoreductase -
EKI57_RS06895 1244876..1245130 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EKI57_RS06900 1245127..1245378 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EKI57_RS06905 1245661..1245708 + 48 WP_001375657.1 his operon leader peptide -
EKI57_RS06910 1245854..1246753 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EKI57_RS06915 1246759..1248063 + 1305 WP_000009589.1 histidinol dehydrogenase -
EKI57_RS06920 1248060..1249130 + 1071 WP_000108914.1 histidinol-phosphate transaminase -
EKI57_RS06925 1249130..1250197 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T120481 WP_000767829.1 NZ_CP037943:c1245130-1244876 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T120481 NZ_CP037943:c1245130-1244876 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT120481 WP_001259255.1 NZ_CP037943:c1245378-1245127 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT120481 NZ_CP037943:c1245378-1245127 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References