Detailed information of TA system TA120465

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 275121..275492
• Replicon: chromosome
• Accession: NZ_CP037943
• Organism: Escherichia coli strain CFSAN027343

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: EKI57_RS01515
• Protein ID: WP_001443846.1
• Coordinates: 275343..275492 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	275121..275299 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI57_RS01490	271076..272449	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
EKI57_RS01495	272578..273513	-	936	WP_096845509.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EKI57_RS01500	273565..274800	-	1236	WP_000040851.1	site-specific integrase	-
EKI57_RS01505	274802..275017	-	216	WP_000079604.1	excisionase XisR	-
EKI57_RS01510	275117..275305	-	189	WP_001302840.1	DUF1187 family protein	-
-	275121..275299	+	179	NuclAT_0	-	Antitoxin
-	275121..275299	+	179	NuclAT_0	-	Antitoxin
-	275121..275299	+	179	NuclAT_0	-	Antitoxin
-	275121..275299	+	179	NuclAT_0	-	Antitoxin
EKI57_RS01515	275343..275492	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EKI57_RS01520	275548..276357	-	810	WP_000166313.1	recombination protein RecT	-
EKI57_RS01525	276350..278950	-	2601	WP_000105150.1	exodeoxyribonuclease VIII	-
EKI57_RS01530	279052..279327	-	276	WP_000632297.1	protein RacC	-
EKI57_RS01535	279402..279572	-	171	WP_001352098.1	conserved protein, Rac prophage	-
EKI57_RS01540	279572..279793	-	222	WP_000560223.1	killing protein KilR	-
EKI57_RS01545	280214..280366	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	273565..365865	92300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T120465 WP_001443846.1 NZ_CP037943:c275492-275343 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T120465 NZ_CP037943:c275492-275343 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT120465 NZ_CP037943:275121-275299 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin