Detailed information of TA system TA120456

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34417..34843
• Replicon: plasmid pCFSAN027350
• Accession: NZ_CP037942
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EKI55_RS27935
• Protein ID: WP_001312861.1
• Coordinates: 34417..34575 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34619..34843 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS27905	29428..29775	+	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
EKI55_RS27910	29795..31366	+	1572	WP_000381416.1	IS66 family transposase	-
EKI55_RS27915	31395..31664	+	270	Protein_30	protein RepA	-
EKI55_RS27920	31814..33352	-	1539	WP_000998048.1	IS66-like element ISEc8 family transposase	-
EKI55_RS27925	33402..33749	-	348	WP_001583081.1	IS66 family insertion sequence element accessory protein TnpB	-
EKI55_RS27930	33746..34126	-	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-
EKI55_RS27935	34417..34575	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	34619..34843	-	225	NuclAT_0	-	Antitoxin
-	34619..34843	-	225	NuclAT_0	-	Antitoxin
-	34619..34843	-	225	NuclAT_0	-	Antitoxin
-	34619..34843	-	225	NuclAT_0	-	Antitoxin
EKI55_RS28940	34655..34843	+	189	WP_001336239.1	hypothetical protein	-
EKI55_RS27940	34855..35574	-	720	WP_032345718.1	plasmid SOS inhibition protein A	-
EKI55_RS27945	35571..36005	-	435	WP_032345719.1	conjugation system SOS inhibitor PsiB	-
EKI55_RS27950	36060..37878	-	1819	Protein_38	ParB/RepB/Spo0J family partition protein	-
EKI55_RS27955	37817..38143	+	327	WP_032345720.1	hypothetical protein	-
EKI55_RS27960	38243..38443	+	201	Protein_40	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / toxB / hlyD / hlyB / hlyA / hlyC	1..157534	157534
	-	inside	IScluster/Tn	-	-	28778..40903	12125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T120456 WP_001312861.1 NZ_CP037942:c34575-34417 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T120456 NZ_CP037942:c34575-34417 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT120456 NZ_CP037942:c34843-34619 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGTCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin