Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3941553..3942055 Replicon chromosome
Accession NZ_CP037941
Organism Escherichia coli strain CFSAN027350

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EKI55_RS20170 Protein ID WP_000767829.1
Coordinates 3941553..3941807 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EKI55_RS20175 Protein ID WP_001259255.1
Coordinates 3941804..3942055 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI55_RS20140 3936568..3936795 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EKI55_RS20145 3936809..3937867 - 1059 WP_000492324.1 transport protein YeeE -
EKI55_RS20150 3938046..3939404 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EKI55_RS20155 3939394..3939456 - 63 WP_010723108.1 membrane protein YoeI -
EKI55_RS20160 3939671..3940600 - 930 WP_000803351.1 LysR family transcriptional regulator -
EKI55_RS20165 3940646..3941470 - 825 WP_000754737.1 SDR family oxidoreductase -
EKI55_RS20170 3941553..3941807 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EKI55_RS20175 3941804..3942055 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EKI55_RS20180 3942338..3942385 + 48 WP_001375657.1 his operon leader peptide -
EKI55_RS20185 3942531..3943430 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EKI55_RS20190 3943436..3944740 + 1305 WP_000009589.1 histidinol dehydrogenase -
EKI55_RS20195 3944737..3945807 + 1071 WP_000108914.1 histidinol-phosphate transaminase -
EKI55_RS20200 3945807..3946874 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T120448 WP_000767829.1 NZ_CP037941:c3941807-3941553 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T120448 NZ_CP037941:c3941807-3941553 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT120448 WP_001259255.1 NZ_CP037941:c3942055-3941804 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT120448 NZ_CP037941:c3942055-3941804 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References